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Interleukin-1 receptor antagonist inhibits calcium accumulation in in vivo chronic granuloma induced by potassium permanganate

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Summary

Interleukin-1 (IL-1) is a monokine that exerts multiple biological activity, including immunity and inflammation. Moreover, IL-1 is involved in Ca2+ release causing hypercalcemia and bone resorption. Recently, a 22 kDa natural inhibitor to IL-1 called interleukin-1 receptor antagonist (IL-1ra) has been described in human fluids, which specifically binds IL-1α or IL-1β receptors. In this study, we found that experimental granuloma induced by subcutaneous injections (0.2 ml) of potassium permanganate (KMnO4) 1:40 saturated crystal solution, after 7 days was strongly inhibited in size, weight and calcium content (measured as dry ash weight by incineration of granuloma tissue) compared with untreated controls, in mice treated intraperitoneally with IL-1ra (20 μg/bolus) given twice; the first at the same time of the induction of the granuloma and the second 24 hours later. In addition, leukotriene B4 and prostaglandin E2 were also inhibited in fresh granuloma of mice treated with IL-1ra. Taken together, these findings conclude for the first time, that the accumulation of calcium in chronic inflammatory states is strongly inhibited by IL-1ra, which decreases tissue calcergy and can potentially be useful for the treatment of calcium-related inflammatory diseases and malignancy-associated hypercalcemia.

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Authors and Affiliations

  1. Immunology Division, Institute of Experimental Medicine, University of Chietti Medical School, Via dei Vestini, 66100, Chieti, Italy

    Pio Conti, Marcella Reale, Maria Rosaria Panara, Fernanda C. Placido & Renato C. Barbacane

  2. Laboratory of Parasitology & Parasitic Diseases, Veterinary Faculty, Aristotle University, 540 06, Thessaloniki, Greece

    Stavros Fridas

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  1. Pio Conti
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  2. Marcella Reale
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  3. Maria Rosaria Panara
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  4. Stavros Fridas
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  5. Fernanda C. Placido
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  6. Renato C. Barbacane
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Conti, P., Reale, M., Panara, M.R. et al. Interleukin-1 receptor antagonist inhibits calcium accumulation in in vivo chronic granuloma induced by potassium permanganate. Calcif Tissue Int 52, 300–304 (1993). https://doi.org/10.1007/BF00296655

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  • DOI: https://doi.org/10.1007/BF00296655

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