Summary
The effects of long-term prostaglandin E2 (PGE2) on cancellous bone in proximal tibial metaphysis were studied in 7-month-old male Sprague-Dawley rats given daily subcutaneous injections of 0, 1, 3, and 6 mg PGE2/kg/day and sacrificed after 60, 120, and 180 days. Histomorphometric analyses were performed on double fluorescent-labeled undecalcified bone specimens. After 60 days of treatment, PGE2 produced diffusely labeled trabecular bone area, increased trabecular bone area, eroded and labeled trabecular perimeter, mineral apposition rate, and bone formation rate at all dose levels when compared with age-matched controls. In rats given PGE2 for longer time periods (120 and 180 days), trabecular bone area, diffusely labeled trabecular bone area, labeled perimeter, mineral apposition, and bone formation rates were sustained at the elevated levels achieved earlier at 60-day treatment. The eroded perimeter continued to increase until 120 days, then plateau. The observation that continuous systemic PGE2 administration to adult male rats elevated metaphyseal cancellous bone mass to 3.5-fold of the control level within 60 days and maintained it for another 120 days indicates that the powerful skeletal anabolic effects of PGE2 can be sustained with continuous administration.
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Ueno K, Woodbury DM, Haba T, Price P, Anderson R, Jee WSS (1984) The effects of prostaglandin E2 in growing rats: Depressed longitudinal and radial growth and increased metaphyseal hard tissue mass. Bone 6:79–86
Furuta Y, Jee WSS (1986) The effect of 16, 16-dimethylprostaglandin E2 on weanling rat skeleton: chronic and systemic administration. Anat Rec 215:305–316
Jee WSS, Ueno K, Deng YP, Woodbury DM (1985) The effects of prostaglandin E2 in growing rats: increased metaphyseal hard tissue and cortico-endosteal bone formation. Calcif Tissue Int 37:148–157
Jee WSS, Ueno K, Kimmel DB, Woodbury DM, Price P, Woodbury LA (1987) The role of bone cells in increasing hard tissue in rapidly growing rats treated with prostaglandin E2. Bone 8:171–178.
Jee WSS, Mori S, Li XJ, Chan S (1990) Prostaglandin E2 enhances cortical bone mass and activates intracortical bone remodeling in intact and ovariectomized female rats. Bone 11:253–266
Mori S, Jee WSS, Li XJ, Chan S, Kimmel DB (1990) Effects of prostaglandin E2 on production of new cancellous bone in the axial skeleton of ovariectomized rats. Bone 11:103–113.
Mori S, Jee WSS, Li XJ (in press) Production of new trabecular bone in osteopenic ovariectomized rats by prostaglandin E2. Calcif Tissue Int
Sone K, Tashiro M, Fujinaga T, Tokayama K, Kurome T (1980) Long-term low-dose prostaglandin E1 administration. J Pediatr 97:866–867
Ueda K, Saito A, Nakano H, Aoshima M, Yokota M, Muraoka R, Iwaya T (1980) Cortical hyperostosis following long-term administration of prostaglandin E2 in infants with cyanotic congenital heart disease. J Pediatr 97:834–836
Ringel RW, Brenner JI, Haney PH, Burns JE, Moulton AL, German MA (1982) Prostaglandin-induced periostitis: a complication of long-term PGE1 infusion in an infant with congenital heart disease. Radiology 142:657–658
Lund JE, Brown WP, Tregerman L (1982) The toxicology of PGE1 and PGI2 In: Wu KK, Rossi EC (eds) Prostaglandin in clinical medicine: cardiovascular and thrombotic disorders. Yearbook Medical Publishers, Inc, New York, pp 93–109
Norrdin RW, Shih MS (1988) Systemic effects of prostaglandin E2 on vertebral trabecular remodeling in beagles used in a healing study. Calcif Tissue Int 42:363–368
Shih MS, Norrdin RW (1987) Effect of PGE2 on regional cortico-endosteal remodeling in beagles with fractured ribs: a histomorphometric study. Bone Miner 3:27–34
High WB (1987) Prostaglandins and bone remodeling. Calcif Tissue Int 41:295–296
Li XJ, Jee WSS, Li LY, Patterson-Buckendahl P (1990) Transient effects of subcutaneously administered prostaglandin E2 on cancellous and cortical bone in young adult dogs. Bone 11:353–364
Hagaman JR, Sanchez TV, Myers RC (1985) The effect of lactation on the mineral distribution profile of the rat femur by single photon absorptiometry. Bone 6:301–306
Jee WSS, Inoue J, Jee KW, Haba T (1983) Histomorphometric assay of the growing long bone. In: Takahashi H (ed) Handbook of bone morphology. Nishimura Co, Ltd, Niigata City, Japan, pp 101–122
Parfitt AM, Drezner MK, Glorieux FH, Kanis JA, Malluche H, Meunier PJ, Ott SM, Recker RR (1987) Bone histomorphometry: standardization of nomenclature, symbols and units. Report of the ASBMR Histomorphometry Committee. J Bone Miner Res 2:595–610
Sokal R, Rohlf FJ (1969) Biometry. Freeman, San Francisco
Li XJ, Jee WSS, Chow SY, Woodbury DM (1990) Adaptation of cancellous bone to aging and immobilization in the rat: a single photon absorptiometry and histomorphometry study. Anat Rec 227:12–24
Jee WSS, Li XJ (1990) Adaptation of cancellous bone to overloading in the adult rat: a single photon absorptiometry and histomorphometry study. Anat Rec 227:418–426
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Ke, H.Z., Jee, W.S.S., Mori, S. et al. Effects of long-term daily administration of prostaglandin-E2 on maintaining elevated proximal tibial metaphyseal cancellous bone mass in male rats. Calcif Tissue Int 50, 245–252 (1992). https://doi.org/10.1007/BF00296289
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DOI: https://doi.org/10.1007/BF00296289