Skip to main content
SpringerLink
Log in

The effect of sodium salicylate on the osteoclast-like cell formation and bone resorption in a mouse bone marrow culture

  • Laboratory Investigations
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Abstract

Salicylates are reported to have an inhibitory effect on bone resorption in vivo and in vitro. The present study examined the effect of sodium salicylate on the formation of osteoclast-like cells in vitro. When mouse bone marrow cells were cultured for 8 days with 10-8 M 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3), numerous clusters of mononuclear and multinucleated cells (MNCs) formed, which stained positive for tartrate-resistant acid phosphatase (TRAP-positive). In similar cultures using sodium salicylate, the number of both TRAP-positive mononuclear and TRAP-positive MNCs were found to diminish in proportion to the concentration of sodium salicylate. A time-course experimental model showed that the number of TRAP-positive MNCs decreased slightly when sodium salicylate was given early in the culture period, and decreased markedly when the drugs were given later in the culture period. Pit formation and bone-resorption area on the bone slices were also inhibited by adding sodium salicylate continuously with 1α,25(OH)2D3. The sodium salicylate showed no cytotoxic effect because the total number of adherent cells, including both TRAP-positive and TRAP-negative cells, was independent of the presence of sodium salicylate. These results suggest that sodium salicylate has an inhibitory effect on the recruitment of osteoclast-like MNCs and that this inhibition is greater during the later stage of mouse bone marrow culture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Vane JR (1971) Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nature (New Biol) 231: 232–235

    Google Scholar 

  2. Klein DC, Raisz LG (1970) Prostaglandins: stimulation of bone resorption in tissue culture. Endocrinology 86:1436–1440

    Google Scholar 

  3. Jee WSS, Ueno K, Deng YP, Woodbury DM (1985) The effects of prostaglandin E2 in growing rats: increased metaphyseal hard tissue and cortico-endosteal bone formation. Calcif Tissue Int 37:148–157

    Google Scholar 

  4. Norrdin RW, Shih MS (1988) Systemic effects of prostaglandin E2 on vertebral trabecular remodeling in beagles used in a healing study. Calcif. Tissue Int 42:363–368

    Google Scholar 

  5. Futami K (1973) Inhibition of bone growth by sodium salicylate. Bull Tokyo Med Dent Univ 20:303–322

    Google Scholar 

  6. Yamada S (1977) Inhibitory action of sodium salicylate on the growth of upper jaw and tibia in rats. Jpn J Pharmacol 27:303–310

    Google Scholar 

  7. Sonobe Y (1981) Studies on the inhibitory action of sodium salicylate on dentine formation in rat incisor. Jpn J Oral Biol 23(4):879–892

    Google Scholar 

  8. Feldman RS, Szeto B, Chauncy HH, Goldhaber P (1983) Nonsteroidal anti-inflammatory drugs in the reduction of human alveolar bone loss. J Clin Periodontol 10:131–136

    Google Scholar 

  9. Williams RC, Jeffcoat MK, Kaplan MC, Goldhaber P, Johnson HG, Wechter WJ (1985) Flurbiprofen: a potent inhibitor of alveolar bone resorption in beagles. Science 227:640–642

    Google Scholar 

  10. Jungkeit MC, Chole RA (1991) Ibuprofen inhibits localized bone resorption in the middle ear. Calcif Tissue Int 48:267–271

    Google Scholar 

  11. Ohya K, Nishimura Y, Ogura H (1990) Inhibition by salicylates of bone resorption and prostaglandin production in mouse calvaria in culture. Tissue Culture Dent 23:15–16

    Google Scholar 

  12. Takahashi N, Yamana H, Yoshiki S, Roodman GD, Mundy GR, Jones SJ, Boyde A, Suda T (1988) Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinology 122 (4):1373–1382

    Google Scholar 

  13. Ibbotson KJ, Roodman GD, McManus LM, Mundy GR (1984) Identification and characterization of osteoclast-like cells and their progenitors in cultures of feline marrow mononuclear cells. J Cell Biol 99:471–480

    Google Scholar 

  14. Roodman GD, Ibbotson KJ, MacDonald BR, Kuehl TJ, Mundy GR (1985) 1α,25-dihydroxyvitamin D3 causes formation of multinucleated cells with several osteoclast characteristics in culture of primate marrow. Proc Natl Acad Sci USA 82:8213–8217

    Google Scholar 

  15. MacDonald BR, Takahashi N, McManus LM, Holahan J, Mundy GR, Roodman GD (1987) Formation of multinucleated cells which respond to osteotropic hormones in long-term human marrow cultures. Endocrinology 120:2326–2333

    Google Scholar 

  16. Burstone MS (1958) Histochemical demonstration of acid phosphatase with naphthol AS-phosphates. J Natl Cancer Inst 21: 523

    Google Scholar 

  17. Minkin C (1982) Bone acid phosphatase: tartrate-resistant acid phosphatase as a marker of osteoclast function. Calcif Tissue Int 34:285–290

    Google Scholar 

  18. Suda T, Shinki T, Takahashi N (1990) The role of vitamin D in bone and intestinal cell differentiation. Annu Rev Nutr 10:195–211

    Google Scholar 

  19. McSheehy PMJ, Chambers TJ (1987) 1,25-Dihydroxyvitamin D3 stimulates rat osteoblastic cells to release a soluble factor that increased osteoclastic bone resorption. J Clin Invest 80:425–429

    Google Scholar 

  20. Bar-Shavit Z, Teitelbaum SL, Reitsma P, Hall A, Pegg LE, Trial J, Kahn AJ (1983) Induction of monocytic differentiation and bone resorption by 1α,25-dihydroxyvitamin D3. Proc Natl Acad Sci USA 80:5907–5911

    Google Scholar 

  21. Takahashi N, Akatsu T, Udagawa N, Sasaki T, Yamaguchi A, Moseley JM, Martin TJ, Suda T (1988) Osteoblastic cells are involved in osteoclast formation. Endocrinology 123(5):2600–2602

    Google Scholar 

  22. Mundy GR, Rodman GD (1987) Osteoclast ontogeny and function. In: Peck WA (ed) Bone and mineral research/5. Elsevier Press, Amsterdam New York Oxford, p 209

    Google Scholar 

  23. Raisz LG, Kream BE, Smith MD, Simmons HA (1980) Comparison of the effects of vitamin D metabolites on collagen synthesis and resorption on fetal rat bone in organ culture. Calcif Tissue Int 32:135–138

    Google Scholar 

  24. Modderman WE, Tuinenburg-Bol Raap AC, Nijweide PJ (1991) Tartrate-resistant acid phosphatase is not an exclusive marker for mouse osteoclasts in cell culture. Bone 12:81

    Google Scholar 

  25. Hattersley G, Chambers TJ (1989) Generation of osteoclastic function in mouse bone marrow cultures: multinuclearity and tartrate-resistant acid phosphatase are unreliable markers for osteoclastic differentiation. Endocrinology 124:1689–1696

    Google Scholar 

  26. Sasaki T, Takahashi N, Higashi S, Suda T (1989) Multinucleated cells formed on calcified dentine from mouse bone marrow cells treated with 1α,25(OH)2D3 have ruffled borders and resorb dentine. Anat Rec 224:379–391

    Google Scholar 

  27. Fuller K, Chambers TJ (1989) Effect of arachidonic acid metabolites on bone resorption by isolated rat osteoclasts. J Bone Miner Res 4:209–215

    Google Scholar 

  28. Udagawa N, Takahashi N, Akatsu T, Tanaka H, Sasaki T, Nishihara T, Koga T, Martin TJ, Suda T (1990) Origin of osteoclast: mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenvironment prepared by bone marrow-derived stromal cells. Proc Natl Acad Sci USA 87:7260–7264

    Google Scholar 

  29. Collins DA, Chambers TJ (1992) Prostaglandin E2 promotes osteoclast formation in murine hematopoietic cultures through an action on hematopoietic cells. J Bone Miner Res 7(5):555–561

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, Faculty of Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, 113, Tokyo, Japan

    A. Soekanto, K. Ohya & H. Ogura

Authors
  1. A. Soekanto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Ohya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Ogura
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Soekanto, A., Ohya, K. & Ogura, H. The effect of sodium salicylate on the osteoclast-like cell formation and bone resorption in a mouse bone marrow culture. Calcif Tissue Int 54, 290–295 (1994). https://doi.org/10.1007/BF00295953

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00295953

Key words

Search

Navigation