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Effect of malathion on nucleic acid synthesis in phytohemagglutinin-stimulated human lymphocytes

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Summary

The effect of malathion, an organophosphorus insecticide, on DNA and RNA synthesis was investigated by measuring the rate of incoporation of 3H thymidine and 3H uridine, respectively, into human lymphocytes stimulated by phytohemagglutinin (PHA). Increasing concentrations of malathion, from 10 to 70 μg/ml, were added to human lymphocyte cultures at different times in relation to PHA introduction. The lowest applied dose of malathion (10 μg/ml) in most cases led to increased incorporation of both 3H thymidine and 3H uridine. Higher concentrations of malathion (30, 50, 70 μg/ml) caused a time- and dosedependent decrease of radioisotope incorporation.

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References

  • Aldridge WN (1971) The nature of the reaction of organophosphorus compounds and carbamates with esterases. Bull WHO 44:25

    PubMed  Google Scholar 

  • Chung RA, Huang I-LO, Brown RW (1967) Studies of DNA, RNA and protein synthesis in HeLa S cells exposed to DDT and dieldrin. J Agric Food Chem 15:497–500

    Google Scholar 

  • Cooper HL (1973) Drugs and the cell cycle. In: Zimmerman AM, Padilla GM, Cameron JL (eds) 1st edn. Academic Press, New York London, pp 148–149

    Google Scholar 

  • Czerski P, Szmigielski S, Litwin J (1966) Simple methods for obtaining purified suspensions of lymphocytes. Vox Sang 11:734–737

    PubMed  Google Scholar 

  • Dean BJ (1972) The mutagenic effects of organophosphorus pesticides on microorganisms. Arch Toxicol 39:67–74

    Google Scholar 

  • Dean BJ (1972) The effect of dichlorvos on cultured human lymphocytes. Arch toxikol 30:75–85

    PubMed  Google Scholar 

  • Dedek W, Lohs KL, Fischer GW, Schmidt R (1976) Alkylation of guanine in mice in vivo by organophosphorus insecticides. I. Trichlorphone and butonate. Pestic Biochem Physiol 6:101–110

    Google Scholar 

  • Filippovich JV, Koshcheenko NN, Romantzev EF (1970) The mechanism of “Biochemical shock.” I. The correlation between the accumulation of some thiol radioprotectors in rat tissues and biochemical changes induced by them. Biochem Pharmacol 19:2533–2539

    Article  PubMed  Google Scholar 

  • Filippovich JV, Sheremetyevskaya TN, Romantzev EF (1971) Mechanism of “Biochemical shock”. II. Direct demonstration of mixed disulfide involvement in the inhibition of deoxycytidine formation in rat thymus in vivo. Biochem Pharmacol 20:135–139

    Article  PubMed  Google Scholar 

  • Fishbein L (1976) Atinopheric mutagens. In: Hollaender A (ed) Chemical mutagens, principles and methods for their detection. 4. Plenum, New York 219–319

    Google Scholar 

  • Forsdyke DR (1973) Serum and lymphocyte activation by phytohaemagglutinin (PHA). Exp Cell Res 77:216–222

    PubMed  Google Scholar 

  • Frawley JP, Fuyat HN, Hagan EG, Blake JR, Fitzhugh OG (1957) Marked potentiation in mammalian toxicity from simultaneous administration of two anticholinesterase compounds. J Pharmacol Exp Ther 121:96–106

    PubMed  Google Scholar 

  • Fukuto TR (1971) Relationships between the structure of organophosphorus compounds and their activity as acetylcholinesterase inhibitors. Bull WHO 44:31

    PubMed  Google Scholar 

  • Gill GR, LaHam QN (1972) Histochemical and radiographic investigations of malathion-induced malformations in embryonic chick limbs. Can J Zool 50:349–351

    PubMed  Google Scholar 

  • Greenberg J, LaHam QN (1969) Malathion-induced teratisms in the developing chick. Can J Zool 47:539–542

    PubMed  Google Scholar 

  • Griffin III DE, Hill WE (1978) In vitro breakage of plasmid DNA by mutagens and pesticides. Mutat Res 52:161–169

    PubMed  Google Scholar 

  • Hersh EM, Oppenheim JJ (1965) Impaired in vitro lymphocyte transformation in Hodgkin's disease. N Engl Med 273:1006

    Google Scholar 

  • Huang CC (1973) Effects on growth but not on chromosomes of the mammalian cells after treatment with three organophosphorus insecticides. Proc Soc Exp Biol Med 142:36–40

    PubMed  Google Scholar 

  • Kalow W, Marton A (1961) Second-generation toxicity of malathion in rats. Nature 192:464–465

    PubMed  Google Scholar 

  • Karczmar AG, Nishi S, Blaber LC (1970) Investigations, particularly by means of anticholinesterase agents, of the multiple peripheral and central cholinergic mechanisms and of their behavioral implications. Acta Vitaminol Enzymol 24:131

    PubMed  Google Scholar 

  • Kimbrough RD, Gaines TB (1968) Effect of organic phosphorus compounds and alkylating agents on the rat fetus. Arch Environ Health 16:805–808

    PubMed  Google Scholar 

  • Lawley PD, Shah SA, Orr DJ (1974) Methylation of nucleic acids by 2,2-dichlorovinyl dimethyl phosphate (dichlorvos, DDVP). Chem Biol Interact 8:171–182

    Article  PubMed  Google Scholar 

  • Löfroth G (1970) Alkylation of DNA by dichlorovos. Naturwissenschaften 57:393–394

    PubMed  Google Scholar 

  • McCann J, Choi E, Yamasaki E, Ames BN (1975) Detection of carcinogens as mutagens in the Salmonella/microsome test: Assay of 300 chemicals. Proc Natl Acad Sci USA 72:5135–5139

    PubMed  Google Scholar 

  • Monjardino JPPV, MacGillivray AJ (1970) RNA and histone metabolism in small lymphocytes stimulated by phytohaemagglutinin. Exp Cell Res 60:1–15

    PubMed  Google Scholar 

  • Murphy SD, DuBois KP (1957) Quantitative measurement of inhibition of the enzymatic detoxification of malathion by EPN (ethyl p-nitrophenyl thionobenzene phosphate). Proc Soc Exp Biol Med 96:813–818

    PubMed  Google Scholar 

  • Myhr BC (1973) A screen for pesticide toxicity to protein and RNA synthesis in HeLa cells. J Agr Food Chem 21:362–367

    Google Scholar 

  • Nicholas AH, Vienne M, Van den Berghe H (1979) Induction of sister-chromatid exchanges in cultured human cells by an organophosphorus insecticide: malathion. Mutat Res 67:167–172

    PubMed  Google Scholar 

  • O'Brien RD (1967) Insecticides. Academic Press, New York

    Google Scholar 

  • O'Brien RD (1969) Phosphorylation and carbamylation of cholinesterase. Ann NY Acad Sci 160:204

    Google Scholar 

  • Rosenberg P, Coon JM (1958) Potentiation between cholinesterase inhibitors. Proc Soc Exp Biol Med 97:836–839

    PubMed  Google Scholar 

  • Rosenkranz HS, Rosenkranz S (1972) Reaction of DNA with phosphoric acid esters: gasoline additive and insecticides. Experientia 28:386–387

    PubMed  Google Scholar 

  • Szickowa AP, Elizarowa ON, Zidkowa LW, Koczjetkow TA, Lewin AJ, Rjazanowa RA, Szjewyrjewa NA (1977) “Metody gigjeniczeskoj i toksykologiczeskoj ocenki biologiczjeskowo djejstwija pesticydow”. Izd Medicina, Moskwa, pp 161–164

    Google Scholar 

  • Tyrsted G, Munch-Petersen B (1977) Early effects of phytohemagglutinin on induction of DNA polymerase, thymidine kinase, deoxyribonucleoside triphosphate pools and DNA synthesis in human lymphocytes. Nucleic Acids Res 4:2713–2723

    PubMed  Google Scholar 

  • Van Bao R, Szabó I, Ruzicska P, Czeizel A (1974) Chromosome aberrations in patients suffering acute organic phosphate insecticide intoxication. Humangenetik 24:33–57

    PubMed  Google Scholar 

  • Walter Z, Czajkowska A, Lipecka K (1980) Effect of malathion on the genetic material of human lymphocytes stimulated by phytohemagglutinin (PHA). Hum Genet 53:375–381

    Article  PubMed  Google Scholar 

  • Walter Z, Grabarczyk M, Kulamowicz I, Oliński R, Wiaderkiewicz R (1979) The influence of malathion on DNA structure isolated from rat liver. Acta Univ Lodziensis, series II, No 23, 3–12

  • Wennerberg R, Löfroth G (1974) Formation of 7-methylguanine by dichlorvos in bacteria and mice. Chem Biol Interact 8:339–348

    Article  PubMed  Google Scholar 

  • Wild D (1975) Mutagenicity studies on organophosphorus insecticides. Mutat Res 32:133–150

    PubMed  Google Scholar 

  • Wright AS, Hutson DH, Wooder MF (1979) The chemical and biochemical reactivity of dichlorvos. Arch Toxicol 42:1–18

    PubMed  Google Scholar 

  • Yoder J, Watson M, Benson WW (1973) Lymphocyte chromosome analysis of agricultural workers during extensive occupational exposure to pesticides. Mutat Res 21:335–340

    PubMed  Google Scholar 

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  1. Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Łódź, Banacha 12/16-90, 237, Łódź, Poland

    A. Czajkowska & Z. Walter

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  1. A. Czajkowska
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  2. Z. Walter
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Czajkowska, A., Walter, Z. Effect of malathion on nucleic acid synthesis in phytohemagglutinin-stimulated human lymphocytes. Hum Genet 56, 189–194 (1980). https://doi.org/10.1007/BF00295693

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  • DOI: https://doi.org/10.1007/BF00295693

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