Summary
Nicotiana sylvestris cell lines resistant to the amino acid analogues S-2-aminoethyl-cysteine (AECR), or 5-methyl-tryptophan (5MTR), were isolated in suspension culture. Assuming these resistances to be dominant, we have attempted to determine if such variant cell lines can be used to select double resistant somatic cell hybrids. A total of 1.8 × 104 control calli from mixed AECR and 5MTR protoplasts, and AECR and 5MTR homokaryotic fusions were placed on double analogue selection, but none survived. Eight somatic hybrid calli (0.8%), able to grow without inhibition on the double analogue selection medium, were obtained after AECR + 5MTR protoplast fusion. These were further determined as hybrids on the basis of resistance level, chromosome number, and chlorophyll content, all characteristics differing in the parental cell lines.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenol-oxidase in Beta vulgaris. Plant Physiol. 24, 1–5 (1949)
Baker, R.M.; Brunette, D.M.; Mankovits, R.; Thompson, L.H.; Whitmore, G.F.; Siminovitch, L.; Till, J.E.: Oubain-resistant mutants of mouse hamster cells in culture. Cell 1, 9–21 (1974)
Carlson, P.S.; Smith, H.H.; Dearing, R.D.: Parasexual interspecific plant hybridization. Proc. Natl. Acad. Sci. (USA) 69, 2292–2294 (1972)
Carlson, P.S.: Methionine sulfoximine-resistant mutants of tobacco. Science 180, 1366–1368 (1973)
Cocking, E.C.; George, D.; Price-Jones, M.J.; Power, J.B.: Selection procedures for the production of interspecies somatic hybrids of Petunia hybrida and Petunia parodii. Albino complementation selection. Plant Sci. Let. 10, 7–12 (1977)
Chaleff, R.S.; Carlson, P.S.: Higher plant cells as experimental organisms. In: Genetic Manipulations with Plant Material. (ed. Ledoux, L.), pp. 351–363. New York: Plenum 1975
Chupeau, Y.; Missonier, G.; Hommel, M.; Goujod, J.: Somatic hybrids of plants by fusion of protoplasts. Observations on the model system ‘Nicotiana glauca-N. langsdorffii’. Molec. gen. Genet. 165, 239–246 (1978)
Dudits, D.; Hadlaczky, G.Y.; Levi, E.; Haydu, Z.S.; Lazar, G.: Somatic hybridization of Daucus carota and D. capillifolius by protoplast fusion. Theor. Appl. Genet. 51, 127–132 (1977)
Gleba, Y.Y.; Butenko, R.G.; Sytnik, K.M.: Fusion of protoplast and the parasexual hybridization of Nicotiana tabacum L. Dokl. Acad. Nauk USSR. 221, 1196–1199 (1975)
Glimelius, K.; Eriksson, T.; Grafe, R.; Muller, A.: Somatic hybridization of nitrate reductase deficient mutants of Nicotiana tabacum by protoplast fusion. Physiol. Plant 44, 273–277 (1978)
Grimsley, H.J.; Ashton, N.W.; Cover, D.J.: Complementation analysis of auxotrophic mutants of the moss Phycomitrella patens using protoplast fusion. Molec. gen. Genet. 155, 103–107 (1977a)
Grimsley, H.J.; Ashton, N.W.; Cover, D.J.: The production of somatic hybrids by protoplast fusion in the moss (Physcomitrella patens). Molec. gen. Genet. 154, 97–100 (1977b)
Harris, M.: Phenotypic expression of drug resistance in hybrid cells. J. Natl. Cancer Inst. 50, 423–429 (1973)
Henshaw, C.G.; Jha, K.K.; Mehta, A.R.; Shakeshaft, D.J.; Street, H.E.: Studies on the growth in culture of plant cells. I. Growth pattern in batch propagated suspension cultures. J. exp. Bot. 17, 562–577 (1966)
Kao, K.N.: Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca. Molec. gen. Genet. 150, 225–230 (1977)
Linsmaier, E.M.; Skoog, F.: Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18, 100–127 (1965)
Maliga, P.: Resistance mutants and their use in genetic manipulation. In: Frontiers of Plant Tissue Culture. London-New York: Academic Press 1978 (in press)
Maliga, P.; Lazar, G.; Joo, F.; Nagy, A.H.; Menczel, L.: Restoration. In: Frontiers of Plant Tissue Culture (ed. Thorpe, T.A.), pp. 381–392. Calgary: University of Calgary Press 1978
Maliga, P.; Kiss, Z.R.; Nagy, A.H.; Lazar, G.: Genetic instability in somatic hybrids of Nicotiana tabacum and Nicotiana knightiana. Molec. gen. Genet. 163, 145–151 (1978)
Melchers, G.; Labib, G.: Somatic hybridization of plants by fusion of protoplasts. I. Selection of light resistant hybrids of ‘haploid’ light sensitive varieties of tobacco. Molec. gen. Genet. 135, 277–294 (1974)
Melchers, G.: Microbial techniques in somatic hybridization by fusion of protoplasts. In: International Cell Biology. (eds. Brinkley, B.R.; Porter, K.R.), pp. 207–215. New York: The Rockefeller University Press 1977
Nagy, J.I.; Maliga, P.: Callus induction and plant regeneration from mesophyll protoplasts of Nicotiana sylvestris. Z. Pflanzenphysiol. 78, 453–455 (1976)
Power, J.B.; Frearson, E.M.; Hayward, C.; George, D.; Evans, P.K.; Berry, S.F.; Cocking, E.C.: Somatic hybridization of Petunia hybrida and P. parodii. Nature 263, 500–502 (1976)
Schieder, O.: Fusionen zwischen protoplasten aus mutanten von Sphaerocarpos donnellii Aust. Biochem. Physiol. Pfl. 165, 433–435 (1974)
Schieder, O.: Hybridization experiments with protoplasts from chlorophylldeficient mutants of some Solanaceous species. Planta 137, 253–257 (1977)
Schieder, O.: Somatic hybrids of Datura innoxia Mill. + Datura discolor Bernh. and Datura innoxia Mill. + Datura stramonium L. var. ‘tatula L.’ Molec. gen. Genet. 162, 113–119 (1978a)
Schieder, O.: Genetic evidence for the hybrid nature of somatic hybrids from Datura innoxia Mill. Planta 141, 333–334 (1978b)
Smith, H.H.; Kao, K.N.; Combatti, N.C.: Interspecific hybridization by protoplast fusion in Nicotiana. J. Hered. 67, 123–128 (1976)
Sunderland, N.: Nuclear cytology. In: Plant tissue and cell culture (ed. Street, H.E.), pp. 161–190. Oxford: Blackwell 1973
Widholm, J.M.: Anthranilate synthetase from 5-methyl-trytophan susceptible and resistant cultured Daucus carota cells. Biochim. Biophys. Acta 279, 48–57 (1972)
Widholm, J.M.: Cultured carrot cell mutants: 5-methyl-tryptophan-resistance trait carried from cell to plant and back. Plant Sci. Let. 3, 323–330 (1974)
Widholm, J.M.: Selection and characterization of cultured carrot and tobacco cells resistant to lysine, methionine, and proline analogs. Can. J. Bot. 54, 1523–1529 (1976)
Widholm, J.M.: Selection and characterization of amino acid analog resistant cell cultures. Crop Sci. 17, 597–600 (1977)
Author information
Authors and Affiliations
Additional information
Communicated by P. Maliga
This study is part of a dissertation by D.W.R.W. in partial fulfillment of the requirements for the Ph. D. degree at the University of Florida
Rights and permissions
About this article
Cite this article
White, D.W.R., Vasil, I.K. Use of amino acid analogue-resistant cell lines for selection of Nicotiana sylvestris somatic cell hybrids. Theoret. Appl. Genetics 55, 107–112 (1979). https://doi.org/10.1007/BF00295435
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00295435