Summary
The use of a combination of transmitted light and epiluminescence after silver and fluorescent staining of chromosome preparations makes it possible to achieve simultaneous visualization of silver-stained NORs and fluorescent chromosomes. This technique permits exact localization of silver precipitates on normal and BrdU-substituted chromosomes. After previous silver impregnation, fluorescent staining by actinomycin-daunomycin-DAPI was used to induce a banding pattern that enables identification of specific chromosomes while observing silver-stained NORs at the same time. Application of this method to Down's syndrome patient revealed a 21/21 Robertsonian translocation with NORs eliminated.
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Buys, C.H.C.M., Osinga, J., Gouw, W.L. et al. Rapid identification of chromosomes carrying silver-stained nucleolus-organizing regions. Hum Genet 44, 173–180 (1978). https://doi.org/10.1007/BF00295410
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DOI: https://doi.org/10.1007/BF00295410