Summary
The locus encoding the human T lymphocyte cell surface antigen Leu-2 has been assigned to chromosome 2 with a DNA mapping panel derived from somatic cell hybrids. The two genomic components identified by a cDNA clone for Leu-2 segregated with human chromosome 2 in all 24 independent hybrid clones examined. The cosegregation of the Leu-2 and immunoglobulin kappa (IgK) loci in hybrids with spontaneous rearrangements of chromosome 2 is consistent with the possibility that the Leu-2 locus is on proximal human 2p near IgK. In the mouse, a locus for a T lymphocyte cell surface antigen with properties similar to Leu-2 is closely linked to the IgK locus on mouse chromosome 6. Hence the syntenic relationship of a gene implicated in T cell killing with the immunoglobulin kappa locus would then be conserved in the mouse and human genomes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF (1984) Leucocyte typing: Human leucocyte differentiation antigens detected by monoclonal antibodies. Springer-Verlag, Berlin
Bootsma D, Kidd KK (1984) Report of the committee on the genetic constitution of chromosomes 2, 3, 4, and 5. Cytogenet Cell Genet 37:22–46
Brook JD, Shaw DJ, Meredith L, Bruns GAP, Harper PS (1984) Localization of genetic markers and orientation of the linkage group on chromosome 19. Hum Genet 68:282–285
Bruns GAP, Leary AC, Eisenman RE, Bazinet CW, Regina VM, Gerald PS (1978) Expression of ACONs and GALT in manrodent somatic cell hybrids. Cytogenet Cell Genet 22:172–176
Bruns GAP, Mintz BJ, Leary AC, Regina VM, Gerald PS (1979) Human lysosomal genes: Arylsulfatase A and beta-galactosidase. Biochem Genet 17:1031–1059
Bruns GAP, Karathanasis SK, Breslow JL (1984) The human apolipoprotein AI-CIII complex is located on chromosome 11. Atherosclerosis 4:97–102
Evans RL, Wall DW, Patsoucas CD, Siegal FP, Fikrig SM, Testa CM, Good RA (1981) Thymus-dependent membrane antigens in man: Inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci USA 78:544–588
Gibson DM, Maclean SJ, Cherry M (1983) Recombination between kappa chain genetic markers and the Lyt-3 locus. Immunogenetics 18:11–116
Gottlieb PD (1974) Genetic correlation of a mouse light chain variable region marker with a thymocyte surface antigen. J Exp Med 140:1432–1437
Hieter PA, Max EE, Seidman JG, Maizel JV, Leder P (1980) Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments. Cell 22:197–207
Kanda N, Schreck R, Alt F, Bruns G, Baltimore D, Latt SA (1983) Isolation of amplified DNA sequences from IMR-32 human neuroblastoma cells: Facilitation by fluorescence-activated flow sorting of metaphase chromosomes. Proc Natl Acad Sci USA 80:4069–4073
Kavathas P, Sukhatme VP, Herzenberg LA, Parnes JR (1984) Molecular cloning of the gene coding for the human T lymphocyte differentiation antigen Leu-2. Proc Natl Acad Sci USA 81:7688–7692
Kohl NE, Kanda N, Schreck RR, Bruns G, Latt SA, Gilbert F, Alt FW (1983) Transposition and amplification of oncogene-related sequences in human neuroblastomas. Cell 35:359–367
Kunkel LM, Tantravahi U, Eisenhard M, Latt SA (1982) Regional localization on the human X of DNA segments cloned from flow sorted chromosomes. Nucleic Acids Res 10:1557–1578
Kurnit DM, Neve RL, Morton CC, Bruns GAP, Ma NSF, Cox D, Klinger HP (1984) Recent evolution of DNA sequence homology in the pericentromeric regions of human acrocentric chromosomes. Cytogenet Cell Genet 38:99–105
Landgren U, Ramstedt U, Axberg I, Ullberg M, Kondal M, Wigzell H (1982) Selective inhibition of human T cell cytotoxicity at levels of target recognition or initiation of lysis by monoclonal OKT3 and Leu-2a antibodies. J Exp Med 155:1579–1584
Latt SA, Willard HF, Gerald PS (1976) BrdU-33258 Hoechst analysis of DNA replication in human lymphocytes with supernumerary or structurally abnormal X chromosomes. Chromosoma 57:135–153
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA (1981) Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med 153:310–323
MacDonald RH, Glasebrook AL, Bron C, Kelso A, Cerottini J-C (1982) Clonal heterogeneity in the functional requirement for Lyt-2/3 molecules on cytolytic T lymphocytes (CTL): Possible implications for the affinity of CTL antigen receptors. Immunol Rev 6:89–115
Malisson B, Rebai N, Mawas C (1982) Inhibition of human T cell mediated cytolysis by monoclonal antibodies to effector cell surface structures. Adv Exp Med Biol 146:563–573
Nakauchi H, Nolan G, Hsu C, Huang H, Kavathas P, Herzenberg LA (1985) Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: Molecular homology to its human counterpart. Leu-2/T8, and to immunoglobulin variable regions. Proc Natl Acad Sci USA (in press)
Nakayama E, Dippol W, Shiku H, Oettgen HF, Old LJ (1980) Allo-antigen-induced T-cell proliferation: Lyt phenotype of responding cells and blocking of proliferation by Lyt antisera. Proc Natl Acad Sci USA 77:2890–2894
Rabbitts TH (1983) The human immunoglobulin genes. Biochem Soc Trans 11:119–126
Reinherz EL, Meuer S, Fitzgerald KA, Hussey RE, Levine H, Schlossman SF (1982) Antigen recognition by human T lymphocytes is linked to surface expression of the T3 molecular complex. Cell 30:735–743
Schwab M, Varmus HE, Bishop JM, Grzeschik K-H, Naylor SL, Sakaguchi AY, Brodeur G, Trent J (1984) Chromosome localization in normal human cells and neuroblastomas of a gene related to c-myc. Nature 306:245–248
Shiloh Y, Brodeur G, Schreck R, Donlon T, Sakai K, Korf B, Shipley J, Bruns G, Seeger R, Latt (1985) Differential amplification of a specific domain of chromosome 2 in untreated neuroblastoma tissues. Proc Natl Acad Sci USA 82:3761–3765
Shinohara N, Sachs DH (1979) Mouse alloantibodies capable of blocking cytotoxic T-cell function. J Exp Med 140:1432–1437
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Sukhatme VP, Vollmer AC, Erickson J, Isobe M, Croce C, Parnes JR (1985) Gene for the human T cell differentiation antigen Leu-2/T8 is closely linked to the light chain locus on chromosome 2. J Exp Med 161:429–434
Wahl GM, Stern M, Stark GR (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci USA 76:3683–3687
Whitehead AS, Bruns GAP, Markham AP, Colten HR, Woods DR (1983) Isolation of human C reactive protein cDNA and localization of the gene to chromosome 1. Science 221:69–71
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bruns, G., Kavathas, P., Shiloh, Y. et al. The human T cell antigen Leu-2 (T8) is encoded on chromosome 2. Hum Genet 70, 311–314 (1985). https://doi.org/10.1007/BF00295366
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00295366