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Transmission electron microscopic studies on the mitotic cycle of nucleolar proteins impregnated with silver

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Abstract

Hela cells were impregnated with silver according to Paweletz et al. (1967). In cells in mitosis not only the nucleolar organizer regions (NORs) are strongly impregnated but also part of the nucleolar material, which accumulates in and around the chromosomes. The treatment with adenosine, which in interphase cells spreads the nucleolar material within the nucleus, also distributes the argentophilic material in and around the chromosomes. During the reconstruction phase this material reassembles around the NORs to form parts of the new nucleolus. The silver impregnation technique clearly demonstrates that two main components are responsible for the argentophily of the nucleolus. This is in agreement with the results obtained by Lischwe et al. (1979).

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Authors and Affiliations

  1. Institute of Cell and Tumor Biology, German Cancer Research Center, D-69, Heidelberg, Federal Republic of Germany

    N. Paweletz & M. C. Risueño

  2. Instituto de Biología Celular, Morfogénesis Celular, Velásquez, 144, 6, Madrid, Spain

    M. C. Risueño

Authors
  1. N. Paweletz
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  2. M. C. Risueño
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Paweletz, N., Risueño, M.C. Transmission electron microscopic studies on the mitotic cycle of nucleolar proteins impregnated with silver. Chromosoma 85, 261–273 (1982). https://doi.org/10.1007/BF00294970

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  • DOI: https://doi.org/10.1007/BF00294970

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