Abstract
With synchronously grown cells of an aerobic unicellular marine cyanobacterium Synechococcus sp. Miami BG 043511, changes in the activities of anoxygenic, nitrogenase-dependent hydrogen photoproduction and oxygen photoevolution were measured under non-growing hydrogen production conditions. Interestingly, synchronously grown cells, incubated in light under an argon atmosphere, exhibited cyclic changes in the activity of nitrogenase-catalyzed hydrogen production for approximately 20- to 25-h intervals. Cyclic photosynthetic oxygen production activity also appeared in approximately the same intervals. However, changes in hydrogen production and oxygen production activities were inversely correlated and temporally separated. Stepwise accumulation of hydrogen in closed vessels was also observed in approximately 24-h cycles in these non-growing cells. These observations in non-growing cells suggest that this unicellular aerobic, nitrogen-fixing cyanobacterium may have an endogenous system to control the exhibition of cyclic rhythms, in addition to the previously studied cell cycle-oriented system. Expression and switching between these two systems may be related to the sufficiency or insufficiency of nitrogen growth nutrients. The possibility of the existence of a common control factor of the two systems involving glycogen is also discussed.
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Literature Cited
Fay P (1992) Oxygen relations of nitrogen fixation in cyanobacteria. Microbiol Rev 56:340–373
Gallon JR (1992) Reconciling the incompatible: N2 fixation and O2. New Phytol 122:571–609
Gallon JR, Perry SM, Rajab TM (1988) Metabolic changes associated with the diurnal pattern of N2 fixation in Gloeothece. J Gen Microbiol 134:3079–3087
Holm-Hansen O, Riemann B (1978) Chlorophyll a determination: improvements in methodology. Oikos 30:438–447
Huang TC, Tu J, Chow TJ, Chen TH (1990) Circadian rhythm of the prokaryote Synechococcus sp. Plant Physiol 92:531–533
Kippert F (1991) Essential clock proteins/circadian rhythms in prokaryotes—what is the evidence? Bot Acta 104:2–4
Kondo T, Strayer CA, Kulkarni RD, Taylor W, Ishiura M, Golden SS, Johnson CH (1993) Circadian rhythms in prokayotes: luciferase as reporter of circadian gene expression in cyanobacteria. Proc Natl Acad Sci USA 90:5672–5676
Kumazawa S, Mitsui A (1985) Comparative amperometric study of uptake hydrogenase and hydrogen photoproduction activities between heterocystous cyanobacterium Anabaena cylindrica B629 and nonheterocystous cyanobacterium Oscillatoria sp. strain Miami BG7. Appl Environ Microbiol 50:287–291
Leon C, Kumazawa S, Mitsui A (1986) Cyclic appearance of aerobic nitrogenase activity during sychronous growth of unicellular cyanobacteria. Curr Microbiol 13:149–153
Mitsui A, Cao S (1988) Isolation and culture of marine nitrogen-fixing unicellular cyanobacterium, Synechococcus. Methods Enzymol 167:105–113
Mitsui A, Kumazawa S (1988) Nitrogen fixation by synchronously growing unicellular aerobic nitrogen-fixing cyanobacteria. Methods Enzymol 167:484–490
Mitsui A, Kumazawa S, Takahashi A, Ikemoto H, Cao S, Arai T (1986) Strategy by which nitrogen-fixing unicellular cyanobacteria grow photoautotrophically. Nature 323:720–722
Mitsui A, Cao S, Takahashi A, Arai T (1987) Growth synchrony and cellular parameters of the unicellular nitrogenfixing marine cyanobacterium, Synechococcus sp. strain Miami BG 043511 under continuous illumination. Physiol Plant 69: 1–8
Mitsui A, Suda S, Hanagata N (1993) Cell cycle events at different temperatures in aerobic nitrogen-fixing marine unicellular cyanobacterium Synechococcus sp. Miami BG 043511. J Mar Biotechnol 1:89–91
Mullineaux PM, Chaplin AE, Gallon JR (1980) Effect of light to dark transition on carbon reserves, nitrogen fixation and ATP concentration in cultures of Gloeocapsa (Gloeothese) sp 1430/3. J Gen Microbiol 120:227–232
Mullineaux PM, Gallon JR, Chaplin AE (1981) Acetylene reduction (nitrogen fixation) by cyanobacteria grown under alternating light-dark cycles. FEMS Microbiol Lett 10:245–247
Mullineaux PM, Chaplin AE, Gallon JR (1983) Synthesis of nitrogenase in the cyanobacterium Gloeothece (Gloeocapsa) sp. CCAP 1430/3. J Gen Microbiol 129:1689–1696
Paerl HVV, Bebout BM (1988) Direct measurement of O2-depleted microzones in marine Oscillatoria: relation to nitrogen fixation. Science 241:442–445
Stal LJ, Bergman B (1990) Immunological characterization of nitrogenase in the filamentous non-heterocystous cyanobacterium Oscillatoria limosa. Planta 182:287–291
Stal LJ, Krumbein WE (1985) Nitrogenase activity, in the non-heterocystous cyanobacterium Oscillatoria sp. grown under alternating light-dark cycles. Arch Microbiol 164:67–71
Suda S, Kumazawa S, Mitsui A (1992) Change in the H2 photoproduction capability in a synchronously grown aerobic nitrogen-fixing cyanobacterium, Synechococcus sp. Miami BG 043511. Arch Microbiol 158:1–4
Sweeney BM, Borgese MB (1989) A circadian rhythm in cell division in a prokaryote, the cyanobacterium, Svnechococcus WH7803. J Phycol 25:183–186
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Mitsui, A., Suda, S. Alternative and cyclic appearance of H2 and O2 photoproduction activities under non-growing conditions in an aerobic nitrogen-fixing unicellular cyanobacterium Synechococcus sp.. Current Microbiology 30, 1–6 (1995). https://doi.org/10.1007/BF00294515
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DOI: https://doi.org/10.1007/BF00294515