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Immunological characterization of hydrogenases in the nitrogen-fixing cyanobacterium Nostoc sp. strain PCC 73102

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Abstract

N2-fixing Nostoc sp. strain PCC 73102 was examined for the presence of hydrogenases. Native-PAGE/immunoblots demonstrated that two proteins with molecular masses of approximately 200 kDa and 215 kDa are immunologically related to hydrogenases purified from Bradyrhizobium japonicum, Azotobacter vinelandii, Methanosarcina barkeri, and Thiocapsa roseopersicina. SDS-PAGE/immunoblots showed that one polypeptide, with a molecular mass of about 58 kDa, is immunologically related to the hydrogenases purified from all the microorganisms mentioned above. In addition, two polypeptides, with molecular masses of approximately 34 and 70 kDa, are immunologically related to the hydrogenases purified from T. roseopersicina and M. barkeri respectively. Immunogold/transmission electron microscopy showed that the hydrogenase proteins are present in both the heterocysts and the vegetative cells.

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Literature Cited

  1. Arp DJ, McCollum LC, Seefeldt LC (1985) Molecular and immunological comparison of membrane-bound H2-oxidizing hydrogenases of Bradyrhizobium japonicum, Alcaligenes europhus, Alcaligenes latus, and Azotobacter vinelandii. J Bacteriol 163:15–20

    Google Scholar 

  2. Asada Y, Kawamura S (1987) Hydrogenase from the unicellular cyanobacterium Microcystis aeroginosa. Phytochemistry 26: 637–640

    Google Scholar 

  3. Carrasco CD, Buettner YA, Golden YW (1995) Programmed DNA rearrangement of a cyanobacterial hupL gene in heterocysts. Proc Natl Acad Sci USA 92:791–795

    Google Scholar 

  4. Chen P-C, Almon H, Böger P (1989) Physiological factors determining hydrogenase activity in nitrogen-fixing heterocystous cyanobacteria. Plant Physiol 89:1035–1038

    Google Scholar 

  5. Daday A, Smith GD (1987) The hydrogenase-nitrogenase relationship in a symbiotic cyanobacterium isolated from Macrozamia communis L. Johnson. Aust J Plant Physiol 14:319–324

    Google Scholar 

  6. Ewart GD, Smith GD (1989a) Purification and properties of soluble hydrogenase from the cyanobacterium Anabaena cylindrica. Arch Biochem Biophys 268:327–337

    Google Scholar 

  7. Ewart GD, Smith GD (1989b) An activity stain for the detection of cyanobacterial hydrogenase in polyacrylamide gels. Biochem Int 19:89–97

    Google Scholar 

  8. Ewart GD, Smith GD (1989c) Immunochemical analysis of the soluble hydrogenase from the cyanobacterium Anabaena cylindrica. Biochim Biophys Acta 997:83–89

    Google Scholar 

  9. Fay P (1992) Oxygen relation of nitrogen fixation in cyanobacteria. Microbiol Rev 56:340–373

    Google Scholar 

  10. Fiebig K, Friedrich B (1989) Purification of the F420-reducing hydrogenase from Methanosarcina barkeri (strain Fusaro). Eur J Biochem 184:79–88

    Google Scholar 

  11. Hallenbeck PC, Benemann JR (1978) Characterization and partial purification of the reversible hydrogenase of Anabaena cylindrica. FEBS Lett 94:261–264

    Google Scholar 

  12. Houchins JP (1984) The physiology and biochemistry of hydrogen metabolism in cyanobacteria. Biochim Biophys Acta 768:227–255

    Google Scholar 

  13. Houchins JP, Burris RH (1981) Occurrence and localization of two distinct hydrogenases in the heterocystous cyanobacterium Anabaena sp. strain 7120. J Bacteriol 146:209–214

    Google Scholar 

  14. Jansson E, Martel A, Lindblad P (1993) Ornithine cycle in Nostoc PCC 73102: Stimulation of in vitro ornithine carbamoyl transferase activity by addition of arginine. Curr Microbiol 26:75–78

    Google Scholar 

  15. Kentemich T, Bahnweg M, Mayer F, Bothe H (1989) Localization of the reversible hydrogenase in cyanobacteria. Z Naturforsch 44c:384–391

    Google Scholar 

  16. Kentemich T, Casper M, Bothe H (1991) The reversible hydrogenase in Anacystis nidulans is a component of the cytoplasmic membrane. Naturwissenschaften 78:559–560

    Google Scholar 

  17. Kovacs KL, Seefeldt LC, Tigyl G, Doyle CM, Mortenson LE, Arp DJ (1989) Immunological relationship among hydrogenases. J Bacteriol 171:430–435

    Google Scholar 

  18. Kumar D, Kumar HD (1990) Effects of some inhibitors and carbon sources on acetylene reduction and hydrogen production of isolated heterocysts of Anabaena sp. (strain CA). Br. Phycol J 25:375–379

    Google Scholar 

  19. Kumar AP, Perraju BTVV, Singh HN (1986) Carbon nutrition and regulation of uptake hydrogenase activity in free-living and symbiotic Anabaena cycadae. New Phytol 104:115–120

    Google Scholar 

  20. Lindbald P (1992) Ornithine cycle in Nostoc PCC 73102. Occurrence and localization of ornithine carbamoyl transferase, and the effects of external carbon and ornithine on nitrogenase activity and citrulline synthesis. Physiol Plant 84:275–282

    Google Scholar 

  21. Lindblad P, Sellstedt A (1991) Immunogold localisation in TEM using uptake hydrogenase as an example. Microsc Anal 26:29–31

    Google Scholar 

  22. Lünsdorf H, Niedrig M, Fiebig K (1991) Immunocytochemical localization of the coenzyme F420-reducing hydrogenase in Methanosarcina barkeri Fusaro. J Bacteriol 137:978–984

    Google Scholar 

  23. Rai AN, Borthakur M, Söderbäck E, Bergman B (1992) Immunogold localization of hydrogenases in the cyanobacterialplant symbioses Peltigera canina, Anthoceros punctatus, and Gunnera magellanica. Symbiosis 12:131–144

    Google Scholar 

  24. Rao KK, Hall DO (1988) Hydrogenases: isolation and assay. Methods Enzymol 167:501–509

    Google Scholar 

  25. Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61

    Google Scholar 

  26. Serebriakova L, Zorin NA, Lindblad P (1994) Reversible hydrogenase in Anabaena variabilis ATCC 29413. Presence and localization in non-N2-fixing cells. Arch Microbiol 161:140–144

    Google Scholar 

  27. Stanier RY, Kunisawa R, Mandel M, Cohen-Bazir G (1971) Purification and properties of unicellular blue-green algae Order Chococcales. Bacteriol Rev 35:171–205

    Google Scholar 

  28. Tredici MR, Margheri MC, De Philippis R, Materassi R (1990) The role of hydrogen metabolism in photoheterotrophic cultures of the cyanobacterium Nostoc sp. strain Cc isolated from Cycas circinalis L J Gen Microbiol 136:1009–1015

    Google Scholar 

  29. Van der Oost J, Cox RP (1989) Hydrogenase activity in nitrate-grown cells of the unicellular cyanobacterium Cyanothece PCC 7822. Arch Microbiol 151:40–43

    Google Scholar 

  30. Vignais PM, Toussaint B (1994) Molecular biology of membrane-bound H2 uptake hydrogenases. Arch Microbiol 161: 1–10

    Google Scholar 

  31. Zorin N, Lindblad P (1993) Localization of hydrogenase in the purple sulphur bacterium Thiocapsa roseopersicina. Arch Microbiol 160:96–100

    Google Scholar 

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Authors and Affiliations

  1. Instituto de Botânica and Centro de Citologia Experimental, Universidade do Porto, Rua do Campo Alegre, 823, 4150, Porto, Portugal

    Paula Tamagnini & Roberto Salema

  2. Department of Physiological Botany, Uppsala University, Villavägen 6, S-752 36, Uppsala, Sweden

    Paula Tamagnini, Fredrik Oxelfelt & Peter Lindblad

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  1. Paula Tamagnini
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  2. Fredrik Oxelfelt
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  3. Roberto Salema
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  4. Peter Lindblad
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Tamagnini, P., Oxelfelt, F., Salema, R. et al. Immunological characterization of hydrogenases in the nitrogen-fixing cyanobacterium Nostoc sp. strain PCC 73102. Current Microbiology 31, 102–107 (1995). https://doi.org/10.1007/BF00294284

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