Skip to main content
Log in

Heterochromatin recognition and analysis of chromosome variation in Scilla sibirica

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

The heteroohromatin of Scilla sibirica, consists of two distinct types: 1) showing enhanced Quinacrine fluorescence and located near the centromere of all the chromosomes of the complement, and 2) with reduced Quinacrine fluorescence and located in various positions along the chromosomes. After a denaturation-reannealing treatment both heterochromatin types are stained by Giemsa, and by acetic-orcein. Acetic-orcein, however, tends to stain preferentially the reduced fluorescence segments. An analysis of chromosome variation in a population of twenty plants, reveals that all the plants are unique in their heterochromatic segment endowment. All the chromosomes are polymorphic but there is a certain constancy for band patterns in individual chromosome types, and for the number of bands per chromosome complement.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Baumann, T. W.: Heterochromatin und DNS-replication bei Scilla sibirica. Exp. Cell Res. 64, 323–330 (1971)

    Google Scholar 

  • Caspersson, T., Farber, S., Foley, G. E., Wagh, U., Zech, L.: Chemical differentiation along metaphase chromosomes. Exp. Cell Res. 49, 214–222 (1968)

    Google Scholar 

  • Ellison, J. R., Barr, H. J.: Quinacrine fluorescence of specific chromosome regions. Late replication and high A-T content in Samoaia leonensis. Chromosoma (Berl.) 36, 357–390 (1972)

    Google Scholar 

  • Heitz, E.: Der Nachweis der Chromosomen. Vergleichende Studien über Zahl, Größe und Form im Pflanzenreich. Z. Bot. 18, 625–681 (1926)

    Google Scholar 

  • La Cour, L. F.: Heterochromatin and the organization of the nucleoli in plants. Heredity 5, 37–50 (1951)

    Google Scholar 

  • Pachmann, U., Riegler, R.: Quantum yield of acridine interaction with DNA of definite base sequences. A basis for the explanation of acridine bands in chromosomes. Exp. Cell Res. 72, 602–608 (1972)

    Google Scholar 

  • Schweizer, D.: Differential staining of plant chromosomes with Giemsa. Chromosoma (Berl.) 40, 307–320 (1973)

    Google Scholar 

  • Vosa, C. G.: Heterochromatin recognition with fluorochromes. Chromosoma (Berl.) 30, 366–372 (1970)

    Google Scholar 

  • Vosa, C. G.: Acetic-orcein, a new stain for the detection of constitutive heterochromatin in plant and animal chromosomes. Exp. Cell Res. (in press, 1973a)

  • Vosa, C. G.: Quinacrine fluorescence analysis of chromosome variation in Tulbaghia leucantha. Chromosomes today 4 (in press, 1973b)

  • Vosa, C. G.: The enhanced and reduced Quinacrine fluorescence bands and their relationship with the Giemsa pattern in Allium flavum. Nobel Symposium 23 (in press, 1973c)

  • Vosa, C. G., Marchi, P.: Quinacrine fluorescence and Giemsa staining in plants. Nature (Lond.) New Biol. 237, 191–192 (1972a)

    Google Scholar 

  • Vosa, C. G., Marchi, P.: On the Quinacrine fluorescence and Giemsa patterns of the chromosomes of Vicia faba. Giorn. bot. Ital. 106, 151–159 (1972b)

    Google Scholar 

  • Weisblum, B., de Haseth, P.: Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythymidylate-rich regions in DNA. Proc. nat. Acad. Sci. (Wash.) 69, 629–632 (1972)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vosa, C.G. Heterochromatin recognition and analysis of chromosome variation in Scilla sibirica . Chromosoma 43, 269–278 (1973). https://doi.org/10.1007/BF00294275

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00294275

Keywords

Navigation