Abstract
Anthers of Lilium candidum L. were cultivated on artificial media containing labelled amino acids. Histones were isolated from meiocytes and fractionated by the use of polyacrylamide gel electrophoresis (PAGE). Total histone synthesis was found not to terminate at the end of premeiotic interphase but to continue until at least zygotene. However, the rate of synthesis was reduced during prophase I compared to interphase. Separate fractions were synthesized asynchronously during the period from late interphase to zygotene. Tissue specific “histone of meiosis” (FM) was synthesized during late interphase and leptotene.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bogdanov, Yu.F., Antropova, E.N.: Delayed termination of nuclear histone doubling after premeiotic DNA synthesis in Triturus vulgaris male meiosis. Chromosoma (Berl.) 35, 353–373 (1971)
Bogdanov, Yu.F., Liapunova, N.A., Sherudilo, A.I., Antropova, E.N.: Uncoupling of DNA and histone synthesis prior to prophase I of meiosis in the cricket Grillus (Acheta) domesticus. Exp. Cell Res. 52, 59–70 (1968)
Georgiev, G.P., Ilyin, Yu.F., Tichonenko, A.S., Stelmashuk, V.Yu.: The structure of chromosomal deoxyribonucleo-proteins. I. The isolation of soluble DNP by urea treatment and its properties. Molec. Biol. (USSR) 4, 246–255 (1970)
Hnilica, L.S.: Proteins of cell nucleus. In: Proc. Nucleic acid research and molecular biology, vol. 7, p. 25–105. London-New York: Academic Press 1967
Hotta, Y., Stern, H.: Analysis of DNA synthesis during meiotic prophase in Lilium. J. Biol. 55, 337–355 (1971)
Johns, E.W.: The electrophoresis of histones in polyacrylamide gel and their quantitative determination. Biochem. J. 104, 78–82 (1967)
Liapunova, N.A., Babadjanian, D.P.: A quantitative study of histon of meiocytes. Investigation of the histone amount in cricket spermatogenesis by interference microscopy. Chromosoma (Berl.) 40, 387–399 (1973)
Phillips, D.M.P.: The primary structure of histones and protamines. In: Histones and nucleohistones (D.M.P. Phillips, ed). vol. 47, p. 47–85. New York-London: Plenum Press 1971
Reznickova, S.A., Bogdanov, Yu.F.: Meiosis in excised anthers of Lilium candidum L. Biol. Zbl. 91, 409–428 (1972)
Reznickova, S.A., Ostroverkhov, V.O.: Cytophotometric study of DNA-content in the process of microsporogeny studied in Lilium candidum L. Dokl. Acad. Nauk. USSR. 194, 695–697 (1970)
Sheridan, W.F., Stern, H.: Histones of meiosis. Exp. Cell Res. 45, 323–335 (1967)
Stern, H., Hotta, Y.: DNA synthesis in relation to chromosome pairing and chiasma formation. Genetics 61, Suppl. 27–39 (1969)
Strokov, A.A., Bogdanov, Yu.F., Reznickova, S.A.: A quantitative study of histones of meiocytes. II. Polyacrylamide gel electrophoresis of isolated histones of Lilium microsporocytes. Chromosoma (Berl.) 43, 247–260 (1973)
Taylor, J.H.: The duration of differentiation in excised anthers. Amer. J. Bot. 37, 137–143 (1950)
Author information
Authors and Affiliations
Additional information
Dedicated to Professor A. A. Prokofieva-Belgovskaia on the occasion of the seventieth anniversary of her birthday.
Rights and permissions
About this article
Cite this article
Bogdanov, Y.F., Strokov, A.A. & Reznickova, S.A. Histone synthesis during meiotic prophase in Lilium . Chromosoma 43, 237–245 (1973). https://doi.org/10.1007/BF00294272
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00294272