Abstract
This study showed that particulate (i.e., physical) toxicity was responsible for rainbow trout deaths in bioassays with two separate solid wastes. This conclusion was based on: (1) fish necropsies which indicated physical damage to gills but no evidence of chemical damage to liver or kidney, (2) chemical analyses which indicated that levels of Priority Pollutants and other target compounds were too low to cause the observed toxicity, (3) structural and chemical analyses of the waste particles which showed that these consisted of inert materials, and (4) the use of centrifugation techniques to remove most of the suspended particulate material in bioassay tanks resulting in an elimination of most of the toxicity. The particles associated with the lethal effects were approximately 5 to 10 μm in size. Regulatory testing of solid wastes must distinguish physical and chemical toxicity since disposal options can vary depending on the mode of toxicity. For instance, chemical toxicity raises concern regarding leaching through soils into groundwater, whereas if physical particles are responsible for toxicity, such leaching is not of concern.
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References
Alabaster, J. S. and Lloyd, R.: 1980, Water Quality Criteria for Freshwater Fish, Butterworths, London, 297 pp.
A.P.H.A.: 1985, Standard Methods for the Examination of Water and Wastewater, 16th edition, APHA/AWWA/WPCF, 1268 pp.
ASTM: 1980, ‘Standard Practice for Conducting Acute Toxicity Tests for Fishes, Macroinvertebrates, and Amphibians’, E 729-80, American Society for Testing and Materials, Philadelphia.
Dowd, R. M.: 1984, Environm. Sci. Technol. 18, 281A.
Hinton, D. E., Kendall, M. W., and Silver, B. B.: 1973, ‘Use of Histologic and Histochemical Assessments in the Prognosis of the Effects of Aquatic Pollutants’, J. Cairns Jr. and K. L. Dickson (eds.), Biological Methods for the Assessment of Water Quality, ASTM STP 528, American Society for Testing and Materials, Philadelphia, pp. 194–205.
Humason, G. L.: 1979, Animal Tissue Techniques, 4th ed., W. H. Freeman and Company, San Francisco, 661 pp.
Mallatt, J.: 1985, Can. J. Fish. Aquat. Sci. 42, 630.
Ongley, E. D.: 1985, personal communication.
Ongley, E. D. and Blachford, D. P.: 1982, Environm. Technol. Lett. 3, 219.
Swartz, R. C., DeBen, W. A., Phillips, J. K., Lamberson, J. O. and Cole, F. A.: 1985, ‘Phoxocephalid Amphipod Bioassay for Marine Sediment Toxicity’, R. D. Cardwell, R. Purdy, and R. C. Balmer (eds.), Aquatic Toxicology and Hazard Assessment: Seventh Symposium, ASTM STP 854, American Society for Testing and Materials, Philadelphia, pp. 284–307.
U.S. EPA: 1972, Water Quality Criteria 1972, EPA-R3-73-033, National Academy of Sciences and National Academy of Engineering, Washington, D.C.
U.S. EPA: 1976, Quality Criteria for Water, Washington, D.C. 256 pp.
U.S. EPA: 1984a, Contract laboratory protocol methodologies IFB WA 84 J091/J092, Washington, D.C. U.S.
EPA: 1984b, Method 1625, Semi-Volatile Organic Compounds by Isotope Dilution Gas Chromatography Mass Spectrometry, 40 CFR Part I36, Federal Register 49, I84.
U.S. EPA: 1985, Water Quality Criteria, Federal Register 50, 30784.
WDOE: 1981, Biological Testing Methods, DOE 80-I2, 18 pp.
Yasutake, W. T. and Wales, J. H.: 1983, Microscopic Anatomy of Salmonids: An Atlas, U.S. Dept. of Interior Publ. 150, Washington, D.C., 189 pp.
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Chapman, P.M., Popham, J.D., Griffin, J. et al. Differentiation of physical from chemical toxicity in solid waste fish bioassays. Water Air Soil Pollut 33, 295–308 (1987). https://doi.org/10.1007/BF00294198
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DOI: https://doi.org/10.1007/BF00294198