Summary
DNA fragments of the fission yeast Schizosaccharomyces pombe that can complement pyrB mutations in Escherichia coli have been cloned into pBR322. Two contiguous HindIII fragments which are 2.7 kb and 1.5 kb in size are essential for this complementation. The cloned fragments have been proved to derive from yeast DNA by the use of Southern hybridization techniques. They apparently carry the structural gene for aspartate transcarbamylase [EC 2.1.3.2] and a promoter signal that is functional in E. coli.
S. pombe is now the fourth eukaryote with a gene shown to be functionally expressed in E. coli. The DNA fragments cloned in this work will be useful in molecular-cloning studies in S. pombe.
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Yamamoto, M., Asakura, Y. & Yanagida, M. Cloning of a gene from the fission yeast S. pombe which complements E. coli pyrB, the gene for aspartate transcarbamylase. Molec. Gen. Genet. 182, 426–429 (1981). https://doi.org/10.1007/BF00293931
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DOI: https://doi.org/10.1007/BF00293931