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Human DNA replication: Fiber autoradiographic analysis of diploid cells from normal adults and from Fanconi's anemia and ataxia telangiectasia

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Summary

Fiber autoradiograms prepared from radioactive DNA of diploid skin fibroblasts and lymphocytes from normal adult humans show patterns of replication of chromosomal DNA that are closely similar to those from other mammalian cells. The rate of replication fork movement is 0.64 μm/min and 0.42 μm/min for fibroblasts and lymphocytes respectively. In both types of cells, replication units show a median length of about 70 μm. Replication proceeds bidirectionally from a central origin in most units and adjacents units initiate synthesis synchronously. In skin fibroblasts from individuals with Fanconi's anemia or ataxia telangiectasia, the autoradiographic patterns of DNA replication are the same as in controls. This suggests that S phase DNA synthesis is normal in these disorders.

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Authors and Affiliations

  1. Department of Medicine, McGill University, 3775 University Street, H3A 2B4, Montreal, Quebec, Canada

    Roger Hand

  2. Department of Microbiology, McGill University, 3775 University Street, H3A 2B4, Montreal, Quebec, Canada

    Roger Hand

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Hand, R. Human DNA replication: Fiber autoradiographic analysis of diploid cells from normal adults and from Fanconi's anemia and ataxia telangiectasia. Hum Genet 37, 55–64 (1977). https://doi.org/10.1007/BF00293772

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  • DOI: https://doi.org/10.1007/BF00293772

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