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Karyotyping of bone-marrow cells in hematologic diseases

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Summary

Cultures of bone-marrow cells incubated for 5 h at 4° had cells in metaphase with elongated chromosomes which were easily Giemsa banded. Colcemid was not necessary for metaphase arrest at this temperature. This technique made possible routine karyotyping of patients with leukemia and other hematological diseases in which the detection of aberrations was otherwise difficult.

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References

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Author notes

  1. J. R. Humbert (Associate Professor of Pediatrics)

    Present address: The Children's Hospital of Buffalo, State University of New York in Buffalo, 219 Bryant Street, 14 222, Buffalo, New York, USA

  2. J. J. Hutter (Assistant Professor of Pediatrics)

    Present address: Arizona Medical Center, University of Arizona, 85 724, Tucson, Arizona, USA

Authors and Affiliations

  1. Department of Biophysics and Genetics, University of Colorado Medical School, 80 262, Denver, Colorado, USA

    H. G. Morse, J. R. Humbert (Associate Professor of Pediatrics), J. J. Hutter (Assistant Professor of Pediatrics) & A. Robinson

  2. Children's Hospital, Denver, Colorado, USA

    H. G. Morse, J. R. Humbert (Associate Professor of Pediatrics), J. J. Hutter (Assistant Professor of Pediatrics) & A. Robinson

  3. National Jewish Hospital and Research Center, 80 206, Denver, Colorado, USA

    H. G. Morse, J. R. Humbert (Associate Professor of Pediatrics), J. J. Hutter (Assistant Professor of Pediatrics) & A. Robinson

Authors
  1. H. G. Morse
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  2. J. R. Humbert
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  3. J. J. Hutter
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  4. A. Robinson
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Morse, H.G., Humbert, J.R., Hutter, J.J. et al. Karyotyping of bone-marrow cells in hematologic diseases. Hum Genet 37, 33–39 (1977). https://doi.org/10.1007/BF00293769

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  • DOI: https://doi.org/10.1007/BF00293769

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