Abstract
The arrangement of repetitive and non-repetitive DNA sequences was studied in the human genome. By Ag+-Cs2SO4 density gradient centrifugations of human DNA at different fragment size reannealed to different C0t values and c-RNA hybridization experiments, we have shown the presence of two repetitive DNA fractions, called fast and slow intermediate DNA, with different pattern of sequence organization. — The fast intermediate DNA sequences (6% of the genome; CsCl density in renatured form: 1.703 g/ml) are in part clustered in fragments greater then 24,000 nucleotide pairs and in part in fragments ranging from 1,800 to 600 nucleotide pairs spaced with longer more complex sequences. — The slow intermediate DNA sequences (30% of the genome; CsCl density in renatured form: 1.707 g/ml) appear to be finely interspersed with non-repetitive sequences. At a DNA fragment size of 600 nucleotide pairs only a third of the slow intermediate DNA sequences are free of unique sequences, while the other two thirds are still organized with unique sequences. — It has also been shown that a great amount of the repetitive DNA sequence transcripts in heterogeneous nuclear RNA of HeLa cells are complementary to slow intermediate DNA sequences.
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Bonner, J., Garrard, W.T., Gottesfeld, J., Holmes, D.S., Sevall, J.S., Wilkes, M.: Functional organization of the mammalian genome. Cold. Spr. Harb. Symp. quant. Biol. 38, 303–310 (1974)
Britten, R.J.: Repeated sequences in human DNA. Carnegie Inst. Yearbook 67, 327–330 (1969)
Britten, R.J., Khone, D.E.: Repeated sequences in DNA. Science (N.Y.) 161, 529–540 (1968)
Cech, T.R., Rosenfeld, A., Hearst, J.E.: Characterization of the most rapidly renaturing sequences in mouse main-band DNA. J. molec. Biol. 81, 299–325 (1973)
Chamberlin, M., Berg, P.: Deoxyribonucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 48, 81–94 (1962)
Chamberlin, M.E., Britten, R.J., Davidson, E.H.: Sequence organization in Xenopus DNA studied by electron microscope. J. molec. Biol. 96, 317–333 (1975)
Corneo, G, Ginelli, E., Polli, E.: Repeated sequences in human DNA. J. molec. Biol. 48, 319–327 (1970a)
Corneo, G., Ginelli, E., Polli, E.: Different satellite deoxyribonucleic acids of guinea pig and ox. Biochemistry 9, 1565–1571 (1970b)
Corneo, G., Ginelli, E., Polli, E.: Renaturation properties and localization in heterochromatin of human satellite DNAs. Biochim. biophys. Acta (Amst.) 247, 528–534 (1971)
Corneo, G., Ginelli, E., Polli, E.: Human leukemic intermediate DNA components. Acta haemat. 54, 210–220 (1975)
Corneo, G., Zardi, L., Polli, E.: Elution of human satellite DNAs on a methylated albumin kieselguhr chromatographic column: isolation of satellite DNA. IV. Biochim. biophys. Acta (Amst.) 269, 201–204 (1972)
Davidson, E.H., Hough, B.R., Amenson, C.S., Britten, R.J.: General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus. J. molec. Biol. 77, 1–23 (1973)
Gillespie, D., Spiegelman, S.: A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. molec. Biol. 12, 829–842 (1965)
Ginelli, E., Corneo, G.: Sui DNA satelliti e ripetitivi del genoma umano. Atti Acc. Naz. Lincei Rc. 53, 621–629 (1972)
Goldberg, R.B., Crain, W.R., Ruderman, J.V., Moore, G.P., Barnett, T.R., Higgins, R.C., Gelfand, R.A., Galau, G.A., Britten, R.J., Davidson, E.H.: DNA sequence organization in the genomes of five marine invertebrates. Chromosoma (Berl.) 51, 225–251 (1975)
Graham, D.E., Neufeld, B.R., Davidson, E.H., Britten, R.J.: Interspersion of repetitive and non-repetitive DNA sequences in the sea urchin genome. Cell 1, 127–137 (1974)
Jelinek, W., Molloy, G., Fernández-Muñoz, R., Salditt, M., Darnell, J.E.: Secondary structure in heterogeneous nuclear RNA: involvement of regions from repeated DNA sites. J. molec. Biol. 82, 361–370 (1974)
Jensen, R.H., Davidson, N.: Spectrophotometric, potentiometric, and density gradient ultracentrifugation studies of the binding of silver ion by DNA. Biopolymers 4, 17–32 (1966)
Jones, K.W.: Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature (Lond.) 223, 582–587 (1970)
Jones, K.W., Corneo, G.: Location of satellite and homogeneous DNA sequences on human chromosomes. Nature (Lond.) New Biol. 233, 268–271 (1971)
Jones, K.W., Prosser, J., Corneo, G., Ginelli, E.: The chromosomal location of human satellite DNA. III. Chromosoma (Berl.) 42, 445–451 (1973)
Jones, K.W., Purdom, I.F., Prosser, J., Corneo, G.: The chromosomal location of human satellite DNA. I. Chromosoma (Berl.) 49, 161–171 (1974)
Manning, J.E., Schmid, C.W., Davidson, N.: Interspersion of repetitive and non-repetitive DNA sequences in the Drosophila melanogaster genome. Cell 4, 141–155 (1975)
Marmur, J.: A procedure for the isolation of deoxyribonucleic acids from micro-organism. J. molec. Biol. 3, 208–218 (1961)
Marmur, J., Doty, P.: Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. molec. Biol. 5, 109–118 (1962)
Melli, M., Ginelli, E., Corneo, G., Di Lernia, R.: Clustering of DNA sequences complementary to repetitive nuclear RNA of Hela cells. J. molec. Biol. 93, 23–38 (1975)
Pagulatos, G.N., Darnell, J.E.: Fractionation of heterogeneous nuclear RNA: rates of hybridization and chromosomal distribution of reiterated sequences. J. molec. Biol. 54, 517–535 (1970)
Sanchez, O., Yunis, Y.Y.: The relationship between repetitive DNA and chromosomal bands in man. Chromosoma (Berl.), 48, 191–202 (1974)
Saunders, G.F., Shirakawa, S., Saunders, P.P., Arrighi, F.E., Hsu, T.C.: Populations of repeated DNA sequences in the human genome. J. molec. Biol. 63, 323–334 (1972)
Studier, F.W.: Sedimentation of the size and shape of DNA. J. molec. Biol. 11, 373–390 (1965)
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Ginelli, E., Corneo, G. The organization of repeated DNA sequences in the human genome. Chromosoma 56, 55–68 (1976). https://doi.org/10.1007/BF00293730
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DOI: https://doi.org/10.1007/BF00293730