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Sizing of the Lactobacillus plantarum genome and other lactic acid bacteria species by transverse alternating field electrophoresis

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Abstract

Pulsed-field gel electrophoresis (transverse alternating field electrophoresis system), combined with rare cutting endonucleases, was used to size the genome of five strains of lactic acid bacteria belonging to the species Lactobacillus plantarum. The sum of the fragment sizes obtained with SfiI restriction digest yielded a total value of 2700–2905 kb. Moreover, SfiI digestion gives specific patterns which could allow an intraspecific identification of L. plantarum strains. The genome sizes of Pediococcus pentosaceus, Pc. acidilactici, and Carnobacterium divergens have been estimated by this method as being 1200 kb, 1560 kb, and 3200 kb respectively.

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Literature Cited

  1. Baril C, Saint Girons I (1990) Sizing of the Leptospira genome by pulsed-field agarose gel electrophoresis. FEMS Microbiol Lett 71:95–100

    Google Scholar 

  2. Blondin B, Vezinhet F (1988) Identification de souches de levures oenologiques par leurs caryotypes obtenus en électrophorèse en champ pulsé. Revue Française d'Oenologie 115: 7–11

    Google Scholar 

  3. Bradbury WC, Murray RGE, Mancini C, Morris VL (1985) Bacterial chromosomal restriction endonuclease analysis of the homology of Bacteroides species. J Clin Microbiol 21:24–28

    Google Scholar 

  4. Chang N, Taylor DE (1990) Use of pulsed-field agarose gel electrophoresis to size genome of Campylobacter jejuni UA580. J Bacteriol 172:5211–5217

    Google Scholar 

  5. Chen H, Keseler IM, Shimkets LJ (1990) Genome size of Myxococcus xanthus determined by pulsed-field gel electrophoresis. J Bacteriol 172:4206–4213

    Google Scholar 

  6. Crameri R, Hintermann G, Hütter R (1983) Deoxyribonucleic acid restriction endonuclease fingerprint characterization of Actinomycete strains. Int J Syst Bacteriol 33:652–655

    Google Scholar 

  7. Daniel P, DeWaele E, Hallet JN (1993) Optimisation of transverse alternating field electrophoresis for strain identification of Leuconostoc oenos. Appl Microbiol Biotechnol 38:638–641

    Google Scholar 

  8. De Man JC, Rogosa M, Sharpe ME (1960) A medium for the cultivation of Lactobacilli. J Appl Bacteriol 23:130–135

    Google Scholar 

  9. Hill HA, Hill JE (1986) The value of plasmid profiling in monitoring Lactobacillus plantarum in silage fermentations. Curr Microbiol 13:91–94

    Google Scholar 

  10. Le Bourgeois P, Mata M, Ritzenthaler P (1989) Genome comparison of Lactococcus strains by pulsed-field gel electrophoresis. FEMS Microbiol Lett 59:65–70

    Google Scholar 

  11. Manachini PL, Parini (1983) DNA restriction endocuclease cleavage patterns, DNA sequence similarity and phenotypical characteristics in some strains of Lactobacillus helveticus and Lactobacillus jugurti. Antonie van Leeuwenhoek 49:143–152

    Google Scholar 

  12. Mielenz JR, Jackson LE, O'Gara F, Shanmugam KT (1979) Fingerprinting bacterial chromosomal DNA with restriction endonuclease EcoRI: comparison of Rhizobium spp. and identification of mutants. Can J Microbiol 25:803–807

    Google Scholar 

  13. Razin S, Harasawa R, Barile M (1983) Cleavage patterns of Mycoplasma chromosome, obtained by using restriction endonucleases, as indicators of genetic relatedness among strains. Int J Syst Bacteriol 33:201–206

    Google Scholar 

  14. Schwarz DC, Saffran W, Welsh J, Haas R, Goldenberg M, Cantor CR (1982) New technique for purifying large DNAs and studying their properties and packaging. Cold Spring Harbor Symp. Quant. Biol 47:189–195

    Google Scholar 

  15. Smith CL, Econome JG, Schutt A, Klco S, Cantor CR (1987) A physical map of the Escherichia coli K12 genome. Science 236:1448–1453

    Google Scholar 

  16. Stahl M, Molin G, Persson A, Ahrne S, Stahl S (1990) Restriction endonuclease patterns and multivariate analysis as a classification tool for Lactobacillus spp. Int J Syst Bacteriol 40:189–193

    Google Scholar 

  17. Tannock GW, Fuller R, Pedersen K (1990) Lactobacillus succession in the piglet digestive tract demonstrated by plasmid profiling. Appl Environ Microbiol 56:1310–1316

    Google Scholar 

  18. Tanskanen EI, Tulloch DL, Hillier AJ, Davidson BE (1990) Pulsed-field gel electrophoresis of SmaI digests of lactococcal genomic DNA, a novel method of strain identification. Appl Environ Microbiol 56:3105–3111

    Google Scholar 

  19. Taylor KA, Barbour AG, Thomas DD (1991) Pulsed-field gel electrophoresis analysis of leptospiral DNA. Infect Immun 59:323–329

    Google Scholar 

  20. Tudor JJ, Marri L, Piggot PJ, Daneo-Moore L (1990) Size of the Streptococcus mutans GS-5 chromosome as determined by pulsed-field gel electrophoresis. Infect Immun 58:838–840

    Google Scholar 

  21. Von Husby KO, Nes IF (1986) Changes in the plasmid profile of Lactobacillus plantarum obtained from commercial meat starter cultures. J Appl Bacteriol 60:413–417

    Google Scholar 

  22. Wards BJ, Collins DM, De Lisle GW (1987) Restriction endonuclease analysis of members of the Mycobacterium avium-M. intracellulare-M. scrofulaceum serocomplex. J Clin Microbiol 25:2309–2313

    Google Scholar 

  23. Whipple DL, Le Febvre RB, Andrews RE, Thiermann AB (1987) Isolation and analysis of restriction endonuclease digestive patterns of chromosomal DNA from Mycobacterium paratuberculosis and other Mycobacterium species. J Clin Bacteriol 25:1511–1515

    Google Scholar 

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  1. SigmO-ITV/LALLEMAND Laboratoire de Biotechnologie, Université de Nantes, 2, rue de la Houssinière, F. 44072, Nantes Cedex 03, France

    Patrice Daniel

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  1. Patrice Daniel
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Daniel, P. Sizing of the Lactobacillus plantarum genome and other lactic acid bacteria species by transverse alternating field electrophoresis. Current Microbiology 30, 243–246 (1995). https://doi.org/10.1007/BF00293640

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