Skip to main content
SpringerLink
Log in

Isolation and partial characterization of uronic acid-containing glycoproteins from Mucor rouxii

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Five different fractions containing uronic acids associated with protein were isolated from the cytoplasm of the filamentous form of Mucor rouxii. A signle fraction was isolated from the cell wall by hot sodium dodecyl sulfate followed by ion exchange column chromatography. Two cytoplasmic entities (peaks I and II) were not adsorbed to DEAE Bio-Gel A. The molecular mass of peaks I to V ranged from 16.5 to 210 kDa. The protein-uronic acid ratios were different for each fraction. The cell wall fraction showed a molecular mass of 16.5 kDa, similar to that of peak II but with differences in chromatographic behavior and protein-uronic acid ratio. The possible role of these molecules as acceptors of sugar residues during polyuronide chain growth is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Andaluz E, Guillen A, Larriba G (1986) Preliminary evidence for a glucan acceptor in the yeast Candida albicans. Biochem J 240:495–502

    Google Scholar 

  2. Andaluz E, Ridruejo JC, Ramírez M, Ruiz-Herrera J, Larriba G (1988) Initiation of glucan synthesis in yeast. FEMS Microbiol Lett 49:251–255

    Google Scholar 

  3. Bartnicki-Garcia S, Lindberg B (1972) Partial characterization of mucoran: the glucoronomanan component. Carbohydr Res 23:75–85

    Google Scholar 

  4. Bartnicki-García S, Nickerson WJ (1962) Induction of yeast-like development in Mucor rouxii by carbon dioxide. J Bacteriol 84:829–840

    Google Scholar 

  5. Bartnicki-García S, Reyes E (1968) Polyuronides in the cell walls of Mucor rouxii. Biochim Biophys Acta 170:54–62

    Google Scholar 

  6. Bartnicki-García S, Lippman E, Heick J (1979) Evidence for a polypeptide acceptor in fungal chitin biosynthesis. Abstr, Annual Meeting American Society of Microbiology, p 106

  7. Bitter T, Muir HM (1962) A modified uronic acid carbazole reaction. Anal Biochem 4:330–334

    Google Scholar 

  8. Blumenfeld ML, Krisman CR (1985) The initiation of glycogen biosynthesis in rat heart. Studies with a purified preparation. J Biol Chem 260:11560–11566

    Google Scholar 

  9. Cohen RE, Ballou CE (1981) Mannoproteins structure. In: Tanner W, Loewus FA (eds) Plant carbohydrates II, vol. 13B. Berlín, Heidelberg: Springer-Verlag, pp 471–478

    Google Scholar 

  10. Dow JM, Carreón RR, Villa VD (1981) Role of membranes of mycelial Mucor rouxii in synthesis and secretion of cell wall matrix polymers. J Bacteriol 145:272–279

    Google Scholar 

  11. Dow JM, Olona PM, Villa VD (1982) Glucuronosyl transferase from the dimorphic fungus Mucor rouxii. Exp Mycol 6:329–334

    Google Scholar 

  12. Elorza MV, Murgui A, Sentandreu R (1985) Dimorphism in Candida albicans: contributions of mannoproteins to the architecture of yeast and mycelial cell walls. J Gen Microbiol 131:2209–2216

    Google Scholar 

  13. Elorza MV, Marcilla A, Sentandreu R (1988) Wall mannoproteins of the yeast and mycelial cell of Candida albicans: nature of the glycosidic bond and polydispersity of their mannan moieties. J Gen Microbiol 132:2393–2403

    Google Scholar 

  14. Elorza MV, Mormeneo S, García de la Cruz F, Gimeno C Sentandreu R (1989) Evidence for the formation of covalent bonds between macromolecules in the domain of the wall of Candida albicans. Biochem Biophys Res Commun 162:1118–1125

    Google Scholar 

  15. Flores-Carreón A, Bartnicki-García S (1982) Polyuronide biosynthesis by cell free extracts of Mucor rouxii. J Gen Microbiol 128:2023–2027

    Google Scholar 

  16. Flores-Carreón A, Balcazar R, Ruiz-Herrera J (1985) Characterization of glucuronosyl transferase from Mucor rouxii: requirement for polyuronide acceptors. Exp Mycol 9:294–301

    Google Scholar 

  17. Laemmli VK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Google Scholar 

  18. Larriba G, Morales M, Ruiz-Herrera J (1981) Biosynthesis of β-glucan microfibrils by cell-free extracts from Saccharomyces cerevisiae. J Gen Microbiol 124:375–383

    Google Scholar 

  19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  20. Marcilla A, Elorza MV, Mormeneo S, Rico H, Sentandreu R (1991) Candida albicans mycelial wall structure: supramolecular complex released by zymoliase, chitinase and β-mercapto-ethanol. Arch Microbiol 155:312–319

    Google Scholar 

  21. Mills GL, Cantino E (1978) The lipid composition of Blasiocladiella emersonii δ-particle and the functions of δ-particle lipid in chitin formation. Exp Mycol 2:99–109

    Google Scholar 

  22. Peberdy JF (1990) Fungal cell walls: a review. In: Kuhn PJ, Trince APJ, Jung MJ, Goosey MW, Copping LG (eds) Biochemistry of cell walls and membranes in fungi. Berlin, Heidelberg: Springer-Verlag, pp 5–30

    Google Scholar 

  23. Ruiz-Herrera J (1992) Fungal cell wall: structure synthesis and assembly. Boca Raton: CRC Press

    Google Scholar 

  24. Sentandreu R, Elorza MV, Mormeneo S, Sanjuan R, Iranzo M (1993) Possible roles of mannoproteins in the construction of Candida albicans cell wall. In: Bossche HV, Odds FC, Kerridge D (eds) Dimorphic fungi in biology and medicine. New York: Plenum pp 169–175

    Google Scholar 

  25. Van Rinsum J, Kliss FM, Van Den Ende H (1991) Cell wall glucomannoproteins of Saccharomyces cerevisiae mnn9. Yeast 7:717–726

    Google Scholar 

  26. Weber K, Osborn M (1969) The reability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem 244:4406–4412

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Microbiologia, Facultat de Farmacia, Universitat de Valencia, Avgda. Vicent Andres Estelles s/n, 46100, Burjassot, Valencia, Spain

    Salvador Mormeneo

  2. Instituto de Investigación en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, CP 36000, Guanajuato, Gto, México

    Roberto Zazueta-Sandoval & Arturo Flores-Carreón

  3. Departamento de Genética y Biología Molecular, CINVESTAV, IPN, Apartado Postal 14-740, CP 07000, México, D.F., México

    Arturo Flores-Carreón

Authors
  1. Salvador Mormeneo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Roberto Zazueta-Sandoval
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Arturo Flores-Carreón
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mormeneo, S., Zazueta-Sandoval, R. & Flores-Carreón, A. Isolation and partial characterization of uronic acid-containing glycoproteins from Mucor rouxii . Current Microbiology 30, 237–241 (1995). https://doi.org/10.1007/BF00293639

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00293639

Keywords

Search

Navigation