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The diameter of chromatin fibres depends on linker length

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Abstract

We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin.

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References

  • Bordas J, Pérez-Grau L, Koch MHJ, Vega MC, Nave C (1986) The superstructure of chromatin and its condensation mechanism. I. Synchrotron radiation X-ray scattering results. Eur Biophys J 13:157–173

    Google Scholar 

  • Cornudella LI, Rocha E (1979) Nucleosome organization during germ cell development in the sea cucumber Holothuria tubulosa. Biochemistry 18:3724–3732

    Google Scholar 

  • Felsenfeld G, McGhee JD (1986) Structure of the 30 nm chromatin fiber. Cell 44:375–377

    Google Scholar 

  • Gerchman SE, Ramakrishnan V (1987) Chromatin higher-order structure studied by neutron scattering and scanning transmission electron microscopy. Proc Natl Acad Sci USA 84:7802–7806

    Google Scholar 

  • Koch MHJ, Vega MC, Sayers Z, Michon AM (1987) The superstructure of chromatin and its condensation mechanism. III. Effect of monovalent and divalent cations X-ray solution scattering and hydrodynamic studies. Eur Biophys J 14:307–319

    Google Scholar 

  • Koch MHJ, Sayers Z, Michon AM, Marquet R, Houssier C, Willführ J (1988) The superstructure of chromatin and its condensation mechanism. V. Effect of linker length, condensation by multivalent cations, solubility and electric dichroism properties. Eur Biophys J 16:177–185

    Google Scholar 

  • Kushida H (1962) A study of cellular swelling and shrinkage during fixation, dehydration and embedding in various standard media. J Electron Microsc 11:135

    Google Scholar 

  • Muñoz-Guerra S, Subirana JA (1982) Cross-linked polyvinyl alcohol: a water soluble polymer as embedding medium for electron microscopy. Mikroskopie 39:346–358

    Google Scholar 

  • Olins AL, Douglas R, Wright EB, Olins DE (1976) Chromatin v bodies: isolation, subfractionation and physical characterization. Nucleic Acids Res 3:3271–3291

    Google Scholar 

  • Phelan JJ, Subirana JA, Cole RD (1972) An unusual group of lysine-rich histones from gonads of a sea cucumber, Holothuria tubulosa. Eur J Biochem 31:63–68

    Google Scholar 

  • Pladellorens M, Subirana JA (1975) Spermiogenesis in the sea cucumber Holothuria tubulosa. J Ultrastruct Res 52:235–242

    Google Scholar 

  • Suau P, Bradbury EM, Baldwin JP (1979) Higher-order structures of chromatin in solution. Eur J Biochem 97:593–602

    Google Scholar 

  • Subirana JA (1970) Nuclear proteins from a somatic and a germinal tissue of the echinoderm Holothuria tubulosa. Exp Cell Res 63:253–260

    Google Scholar 

  • Subirana JA, Mũnoz-Guerra S, Aymami J, Radermacher M, Frank J (1985) The layered organization of nucleosomes in 30 nm chromatin fibres. Chromosoma 91:377–390

    Google Scholar 

  • Thoma F, Koller TH, Klug A (1979) Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructure of chromatin. J Cell Biol 83:403–427

    Google Scholar 

  • Van Holde KE, Allen JR, Corden J, Lohr D, Tatchell K, Weischet WO (1979) Nuclease digestion and the structure of chromatin. In: Nicolini CA (ed) Chromatin structure and function. Plenum Press, New York, p 389

    Google Scholar 

  • Williams SP, Athey BD, Muglia LJ, Schappe RS, Gough AH, Langmore JP (1986) Chromatin fibres are left-handed double helices with diameter and mass per unit length that depend on linker length. Biophys J 49:233–248

    Google Scholar 

  • Woodcock CLF, Frado LL, Rattner JB (1984) The higher-order structure of chromatin. Evidence for a helical ribbon arrangement. J Cell Biol 99:42–52

    Google Scholar 

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Alegre, C., Subirana, J.A. The diameter of chromatin fibres depends on linker length. Chromosoma 98, 77–80 (1989). https://doi.org/10.1007/BF00293338

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  • DOI: https://doi.org/10.1007/BF00293338

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