Skip to main content
SpringerLink
Log in

Characterization of Parasarcophaga heterochromatin

II. Polytene chromosomes

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

The polytene chromosomes of six species of Parasarcophaga were examined by C-, quinacrine (Q)-, Hoechst 33258 (H)-, Hoechst/actinomycin D (H/AMD)- and acridine orange (AO)- staining methods to characterize their heterochromatin. In the polytene karyotype the sex chromosomes do not polytenize and are represented by a granular mass of heterochromatin which is C+, Q+, H+ and fluoresces yellowish green with acridine orange. The autosomal elements reveal an identical distribution of heterochromatin with all the banding techniques and the fluorescence characteristics indicate AT-rich fast-reassociating DNA in these regions. Thus, in the polytene system of Parasarcophaga besides the underreplicative heterochromatin there is a considerable amount of heterochromatin which seems to be correplicative during polytenization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Agrawal UR, Kaul D (1988) Polytene chromosome maps of Parasarcophaga albiceps (Sarcophagidae:Diptera). In: Bhatnagar VS (ed) Advances in cytology and genetics. Allahabad Univ, Allahabad, pp 76–88

    Google Scholar 

  • Bedo DG (1975) C-banding in polytene chromosomes of Simulum ornatipes and S. melatum (Diptera:Simulidae). Chromosoma 51:291–300

    Google Scholar 

  • Bedo DG (1986) Polytene and mitotic chromosome analysis in Ceratitis capitata (Diptera: Tephritidae). Can J Genet Cytol 28:180–188

    Google Scholar 

  • Bultmann H, Mezzanotte R (1987) Characterization and origin of extrachromosomal DNA granules in Sarcophaga bullata. J Cell Sci 88:327–334

    Google Scholar 

  • Childress D (1969) Polytene chromosomes and linkage group-chromosome correlations in the Australian sheep blowfly Lucilia cuprina (Diptera:Calliphoridae). Chromosoma 26:208–214

    Google Scholar 

  • Das BC, Raman R, Sharma T (1979) Chromosome condensation and Hoechst 33258 fluorescence in meiotic chromosomes of the grasshopper Spathosternum prasiniferum (Walker). Chromosoma 70:251–258

    Google Scholar 

  • Foster GG, Whitten MJ, Konovalov C, Bedo DG, Maddern RH, Boon DJ (1980) Cytogenetic studies of Lucilia cuprina dorsalis R.D. (Diptera:Calliphoridae). Polytene chromosome maps of the autosomes and cytogenetic localization of visible genetic markers. Chromosoma 81:151–168

    Google Scholar 

  • Gaur P, Agrawal UR, Tewari RR, Kaul D (1985) C- and Q-band polymorphism in the mitotic chromosomes of flesh-flies belonging to the genus Parasarcophaga (Sarcophagidae:Diptera). La Kromosomo II-39-40:1207–1213

    Google Scholar 

  • Hägele K (1979) Selective staining of X-chromosome segments in Schistocerca gregaria after denaturation and reassociation procedures. Chromosoma 71:217–225

    Google Scholar 

  • Jorgenson KF, Sande JH van de, Lin CC (1978) The use of basepair specific DNA binding agents as affinity labels for the study of mammalian chromosomes. Chromosoma 68:287–302

    Google Scholar 

  • Kaul D, Tewari RR (1978) Polytene chromosomes of the fleshfly Parasarcophaga argyrostoma (Sarcophagidae:Diptera). Genetica 49:189–197

    Google Scholar 

  • Kaul D, Tewari RR (1983) Interspecific comparisons of polytene chromosomes in the genus Parasarcophaga (Sarcophagidae:Diptera). Genetica 62:129–138

    Google Scholar 

  • Kaul D, Chaturvedi R, Gaur P, Tewari RR (1978) Cytogenetics of the genus Parasarcophaga (Sarcophagidae:Diptera). Chromosoma 68:75–82

    Google Scholar 

  • Kaul D, Agrawal UR, Tewari RR (1983) Puffing activity in the foot pad chromosomes of Parasarcophaga ruficornis (Fab.) (Sarcophagidae:Diptera). La Kromosomo II-32:961–970

    Google Scholar 

  • Kaul D, Gaur P, Agrawal UR, Tewari RR (1989) Characterization of Parasarcophaga heterochromatin. I. Mitotic chromosomes. Chromosoma 98:49–55

    Google Scholar 

  • Lakhotia SC, Mishra A (1980) Fluorescence patterns of heterochromatin in mitotic and polytene chromosomes in seven members of three subgroups of the melanogaster species group of Drosophila. Chromosoma 81:137–150

    Google Scholar 

  • Lee CS, Collins L (1977) Q- and C-bands in the metaphase chromosomes of Drosophila nasutoides. Chromosoma 61:57–60

    Google Scholar 

  • Lemeunier F, Dutrillaux B, Ashburner M (1978) Relationships within the melanogaster subgroup species of the genus Drosophila (Sophophora). III. The mitotic chromosomes and quinacrine fluorescent patterns of the polytene chromosomes. Chromosoma 69:349–361

    Google Scholar 

  • Pell PE, Southern DI (1976) A cytogenetical study of Glossina fuscipes fuscipes including a comparison of the polytene chromosome maps with those of Glossina austeni. Genetica 46:511–528

    Google Scholar 

  • Ramachandra NB, Ranganath HA (1985) Supernumerary chromosomes in Drosophila nasuta albomicana. Experientia 41:680–681

    Google Scholar 

  • Ramachandra NB, Ranganath HA (1986) Estimation of population fitness in two strains of Drosophila nasuta albomicana with and without supernumerary chromosomes. Indian J Exp Biol 24:137–141

    Google Scholar 

  • Ribbert D (1967) Die Polytänchromosomen der Borstenbildungszellen von Calliphora erythrocephala unter besonderer Berücksichtigung der Puffmuster während der Metamorphose. Chromosoma 21:296–344

    Google Scholar 

  • Roberts B (1968) DNA granule synthesis in the giant foot pad nuclei of Sarcophaga bullata. J Cell Biol 39:112a

    Google Scholar 

  • Roberts B (1975) The volume and DNA content of extrachromosomal inclusions in the dorsal foot pad nuclei of Tricholioproctia impatiens (Sarcophagidae:Diptera). Chromosoma 52:49–58

    Google Scholar 

  • Roberts B, Roberts S (1972) The DNA content of testes and Malpighian tubules of Tricholioproctia impatiens (Sarcophagidae: Diptera). Chromosoma 39:83–91

    Google Scholar 

  • Samols D, Swift H (1979a) Genomic organization in the flesh fly Sarcophaga bullata. Chromosoma 75:129–143

    Google Scholar 

  • Samols D, Swift H (1979b) Characterization of extra chromosomal DNA in the flesh fly Sarcophaga bullata. Chromosoma 75:145–159

    Google Scholar 

  • Schmidt ER (1980) Two AT-rich satellite DNAs in the chironomid Glyptotendipes barbipes (Staeger). Isolation and localization in polytene chromosomes of G. barbipes and Chironomus thummi. Chromosoma 79:315–328

    Google Scholar 

  • Sharma GP, Handa SM, Kaur P, Chopra K (1979) Mapping of the salivary gland chromosomes of Musca domestica (Muscidae: Diptera). Cytologia 44:541–547

    Google Scholar 

  • Southern DI, Pell PE (1974) Comparative analysis of polytene chromosomes of Glossina austeni and Glossina morsitans morsitans. Chromosoma 47:213–226

    Google Scholar 

  • Srivastava US, Shukla KM, Kaul D, Tewari RR (1982) The polytene chromosomes of Parasarcophaga ruficornis (Fab.) (Sarcophagidae: Diptera). Proc Natl Acad Sci (India) 52(B)II: 155–164

    Google Scholar 

  • Steinemann M (1978) Co-replication of satellite DNA of Chironomus melanotus with mainband DNA during polytenization. Chromosoma 66:127–139

    Google Scholar 

  • Swift H, Samols D, Roberts B, Brisard D (1978) Extrachromosomal DNA in polytene nuclei of Sarcophaga. In: Dirksen ER, Prescott DM, Fox CF (eds) Cell reproduction, Academic Press, New York, pp 173–185

    Google Scholar 

  • Tewari RR, Kurahashi H (1985) The karyotype of Helicophagella melanura (Meigen) (Sarcophagidae:Diptera). CIS 38:14–15

    Google Scholar 

  • Tewari RR, Agrawal UR, Kaul D (1983) Heterochromatin polymorphism in Parasarcophaga misera (Sarcophagidae:Diptera). Nucleus 26(3): 188–191

    Google Scholar 

  • Tewari RR, Kurahashi H, Rai V (1986) Fluorescence patterns of mitotic and polytene chromosomes of the genus Boettcherisca (Sarcophagidae:Diptera). IInd European Congress on Cell Biology, Budapest, Hungary, p 6

  • Trepte H-H (1976) Das Puffmuster der Borstenapparat-Chromosomen von Sarcophaga barbata. Chromosoma 55:137–164

    Google Scholar 

  • Trepte H-H (1977) Über die Beziehungen zwischen dem Wachstum der Kerne und dem Wachstum und der Puffaktivität der Polytänchromosomen während der Entwicklung des Borstenapparates bei der Fleischfliege Sarcophaga barbata. Biol Zentralbl 96:551–570

    Google Scholar 

  • Vecchi ML, Rubini PG (1973) Polytene chromosomes of trichogen cells in Musca domestica. I. Genet Agrar 24:456–463

    Google Scholar 

  • Walter L (1973) Syntheseprozesse an den Riesenchromosomen von Glyptotendipes. Chromosoma 41:327–360

    Google Scholar 

  • Whitten JM (1965) Differential deoxyribonucleic acid replication in the giant foot pad cells of Sarcophaga bullata. Nature 208:1019–1021

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Zoology, University of Allahabad, 211 002, Allahabad, India

    Dipika Kaul, Uma Rani Agrawal & Raghav Ram Tewari

Authors
  1. Dipika Kaul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Uma Rani Agrawal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Raghav Ram Tewari
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaul, D., Agrawal, U.R. & Tewari, R.R. Characterization of Parasarcophaga heterochromatin. Chromosoma 98, 56–63 (1989). https://doi.org/10.1007/BF00293335

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00293335

Keywords

Search

Navigation