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In situ nick translation of metaphase chromosomes with biotin-labeled d-UTP

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Summary

Limited nick translation experiments on fixed chromosomes were performed. Sites of preferential DNase-I nicking were made visible by the incorporation of biotin-labeled dUTP and subsequent binding of the streptavidin-peroxidase complex. This procedure leads to a banding pattern on the chromosomes which is strongly DNase-I concentration dependent. Along the chromosome arms, regions of enhanced DNase-I sensitivity alternate with regions of lower DNase-I sensitivity. No complete G- or R-type banding pattern was observed.

The easily identifiable human Y chromosome was studied more intensively. Compiled data show the heterochromatin of the Y chromosome stained as heavily as the euchromatin. The boundary between the eu- and heterochromatin on the long arm appears to be a site of preferential DNase-I sensitivity.

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Authors and Affiliations

  1. Abteilung Klinische Genetik, Universität Ulm, Oberer Eselsberg, D-7900, Ulm, Federal Republic of Germany

    Sabine Adolph & Horst Hameister

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  1. Sabine Adolph
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  2. Horst Hameister
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Adolph, S., Hameister, H. In situ nick translation of metaphase chromosomes with biotin-labeled d-UTP. Hum Genet 69, 117–121 (1985). https://doi.org/10.1007/BF00293280

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  • DOI: https://doi.org/10.1007/BF00293280

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