Abstract
The composition in segregated haploid sets of paternal and maternal chromosomes has been studied in order to verify whether their composition is uniparental of mixed, fixed or variable. Primary cultures where prepared using kidneys from hybrids of strains of Mus musculus in which the parental chromosomes are distinguishable; the maternal set consists of 20 teleocentric chromosomes, the paternal set of 9 metacentric chromosomes, derived by Robertsonian fusion and 2 telocentrics. Applying Seabright's banding technique, an analysis of segregated haploid and diploid cells, which have originated spontaneously through polyploidisation-segregation processes was carried out. It was concluded that the haploid sets have a variable composition of paternal and maternal chromosomes.
Similar content being viewed by others
References
Capanna, E., Civitelli, M.V., Cristaldi, M.: Una popolazione appenninica di Mus musculus L. caratterizzata da un cariotipo a 22 chromosomi. Rend. Acc. Naz. Lincei, Ser. VIII, 54, 981–985 (1973)
Capanna, E., Cristaldi, M., Perticone, P., Rizzoni, M.: Identification of chromosomes involved in the 9 Robertsonian fusion of the Apennine mouse with a 22-chromosome karyotype. Experientia (Basel) 31, 294–296 (1975)
Hullèn, M., Karlaman, A., Lindsten, J., Tiepolo, L.: Aneuploidy and polyploidy in Chinese hamster (Cricetulus griseus). Hereditas (Lund) 65, 197–202 (1970)
Martin, C.M., Sprague, C.A.: Parasexual cycle in cultivated Human somatic cells. Science 166, 761–763 (1970)
Moorhead, P.S., Nowell, P.C., Mellman, W.S., Battips, D.M., Hungerford, D.A.: Chromosomes preparations of leukocyte cultures from human perpheral blood. Exp. Cell Res. 20, 613–616 (1960)
Moscona, A.: Cell suspension from organ rudiments of chicken embryos. Exp. Cell Res. 3, 535–548 (1952)
Palitti, F., Rizzoni, M.: Pattern of DNA segregation in multipolar anatelophases of different ploidy in euploid and aneuploid mammalian cells cultivated in vitro. Genetica (den Haag) 43, 130–147 (1972)
Pawlowitzki, I.H., Cenani, A.: Sporadic triploid cells in human blood and fibroblast cultures. Humangenetic 5, 65–69 (1967)
Pera, F., Schwarzacher, H.G.: Die Verteilung der Chromosomen auf die Tochterzellkerne multipolarer Mitosen in euploiden Gewebekulturen von Microtus agrestis. Chromosoma (Berl.) 26, 337–354 (1969)
Pera, F., Rainer, B.: Studies of multipolar mitosis in euploid tissue cultures. I Somatic reduction to exactly haploid and triploid chromosome sets. Chromosoma (Berl.) 42, 71–80 (1973)
Rizzoni, M., Palitti, F., Perticone, P.: Euploid segregation through multipolar mitosis in mammalian cell cultures. Identification of triploid, haploid and segregating diploid cells in a diploid-euploid primary culture of Rhesus kidney cells. Chromosoma (Berl.) 45, 151–162 (1974)
Seabright, M.: The use of proteolytic enzymes for the mapping of structural rearrangements on the chromosomes of man. Chromosoma (Berl.) 36, 204–210 (1972)
Sinha, A.K.: Spontaneous occurrence of tetraploid and near haploid cells in mammalian peripheral blood. Exp. Cell Res. 47, 443–448 (1967)
Tepliz, R.L., Gustafson, P.E., Pellet, O.L.: Chromosomal distribution in interspecific in vitro hybrid cells. Exp. Cell Res. 52, 379–391 (1968)
Wurster, D.H.: Mouse chromosomes identified by trypsin-Giemsa (T-G) banding. Cytogenetics 11, 379–387 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Perticone, P., Tanzarella, C., Palitti, F. et al. Chromosome constitution of in vitro segregated haploid and diploid cells of the mouse. Chromosoma 56, 243–248 (1976). https://doi.org/10.1007/BF00293188
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00293188