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Étude de mutants thermosensibles du virus Sigma

Characterization of two temperature-sensitive mutants of Sigma virus

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Summary

Temperature-sensitive mutants have been isolated from Drosophila Sigma virus, a Rhabdovirus inducing CO2 sensitivity in Drosophila melanogaster. We have studied the decay of infectious centers at non permissive temperature. The proportion of destroyed infectious centers is the same for the wild type, ts+, and for ts9. On the opposite, it is more important for ts 4. Temperature-sensitive function of ts 4 appears necessary to the viral genome replication. With the three clones, ts+, ts 4 and ts 9, we have obtained “stabilized” Drosophila females able to transmit Sigma virus to their whole progeny. We have tried to see in each case, if stabilized flies could transmit the virus to their progeny at non permissive temperature. Flies stabilized with ts+ and ts 9 can, flies stabilized with ts 4 cannot. Therefore two categories of mutants are defined: those that are transmitted hereditarily. at non permissive temperature, and not blocked in genome replication. Those that are blocked in genome replication and not transmitted. When the virus cannot replicate, the divisions in the germ line cells dilute the viral genomes. The consequence will be a real healing of germ line cells, and then a break in hereditary transmission by stabilized flies. All the results with temperature-sensitive mutants are coherent with this hypothesis.

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Bibliographie

  • Baltimore, D., Huang, A. S., Stampfer, M.: Ribonucleic acid synthesis of vesicular virus. II.—an RNA polymerase in the virion. Proc. nat. Acad. Sci. (Wash.) 66, 572–576 (1970).

    Google Scholar 

  • Berkaloff, A., Bregliano, J. C., Ohanessian, A.: Mise en évidence de virions dans des drosophiles infectées par le virus héréditaire σ. C.R. Acad. Sci. (Paris) 260, 5956–5959 (1965).

    Google Scholar 

  • Bernard, J.: Contribution à l'étude du virus héréditaire de la Drosophile, Sigma. Sa propagation au niveau de disques imaginaux transplantés dans des adultes. Exp. Cell Res. 50, 117–126 (1968).

    Google Scholar 

  • Bernard, J.: Propagation d'un virus Sigma défectif au niveau de disques imaginaux d'aile implantés dans des Drosophiles adultes. J. gen. Virol. 8, 209–218 (1970).

    Google Scholar 

  • Bishop, D. H. L., Roy, P.: Kinetics of RNA synthesis by vesicular stomatitis virus particles. J. molec. Biol. 57, 513–527 (1971).

    Google Scholar 

  • Brun, G.: Etude d'une association du virus σ et de son hôte, la Drosophile: l'état stabilisé. Thèse, 1963, Lab. de Génétique des Virus, 91-Gif sur Yvette (France).

  • Brun, G., Sigot, A.: Etude de la sensibilité héréditaire au gaz carbonique chez la Drosophile. II. —Installation du virus σ dans la lignée germinale à la suite d'une inoculation. Ann. Inst. Pasteur 88, 488–513 (1955).

    Google Scholar 

  • Bussereau, F.: Etude du symptôme de la sensibilité au CO2 produit par le virus Sigma chez la Drosophile. I.—Influence du lieu d'inoculation sur le délai d'apparition du symptôme. Ann. Inst. Pasteur 118, 367–385 (1970a).

    Google Scholar 

  • Busserau, F.: Etude du symptôme de la sensibilité au CO2 produit par le virus Sigma chez la Drosophile. II.—Evolution comparée du rendement des centres nerveux et de divers organes après inoculation dans l'abdomen et dans le thorax. Ann. Inst. Pasteur 118, 626–645 (1970b).

    Google Scholar 

  • Bussereau, F.: Etude du symptôme de la sensibilité au CO2 produit par le virus de la stomatite vésiculaire chez Drosophila melanogaster. I.—VSV de sérotype New-Jersey et le virus Cocal. Ann. Inst. Pasteur 121, 223–239 (1971).

    Google Scholar 

  • Bussereau, F.: Etude du symptôme de la sensibilité au CO2 produit par le virus de la stomatite vésiculaire chez Drosophila melanogaster. II.—VSV de sérotype Indiana. Ann. Inst. Pasteur 122, 1029–1058 (1972).

    Google Scholar 

  • Flamand, A.: Etude génétique du virus de la stomatite vésiculaire classement de mutants thermosensibles spontanés en groupes de complémentation. J. gen. Virol. 8, 187–195 (1970).

    Google Scholar 

  • Gomatos, P. J.: Reovirus-specific, single stranded RNA's synthesized in vitro with enzyme purified from reovirus-infected cells. J. molec. Biol. 37, 423–439 (1968).

    Google Scholar 

  • Gomatos, P. J.: Comparison of the virion polymerase of reovirus with the enzyme purified from reovirus-infected cells. J. Virol. 6, 610–620 (1970).

    Google Scholar 

  • Lafay, F.: Etude des mutants thermosensibles du virus de la stomatite vésiculaire (VSV): classification de quelques mutants d'après des critères de fonctionnement. C. R. Acad. Sci. (Paris) 268, 2385–2388 (1969).

    Google Scholar 

  • Lafay, F., Berkaloff, A.: Etude des mutants themosensibles du virus de la stomatite vésiculaire (VSV): mutants de maturation. C.R. Acad. Sci. (Paris) 269, 1031–1034 (1969).

    Google Scholar 

  • Lestrange, M. Th. de: Contribution à l'étude dc virus héréditaire de la Drosophile: action de l'hyperthermie sur le contenu en virus des tissus somatiques de l'hôte. Ann. Génét. 6, 39–96 (1963).

    Google Scholar 

  • Lewis, E. B., Bacher, F.: Method of feeding ethyl methane sulfonate (EMS) to Drosophila males. DIS 43, 193 (1968).

    Google Scholar 

  • L'Heritier, Ph.: The hereditary virus of Drosophila. Advanc. Virus Res. 5, 195–245 (1958).

    Google Scholar 

  • Martin, E. M.: Studies on the polymerase of some temperature sensitive mutants of Semliki Forest Virus. Virology 39, 107–117 (1969).

    Google Scholar 

  • Ohanessian, A.: Sigma virus multiplication in Drosophila cell lines of different genotypes. Curr. Top. Microbiol. Immunol. 55, 230–233 (1971).

    Google Scholar 

  • Ohanessian-Guillemain, A.: Etude de facteurs génétiques contrôlant les relations du virus σ et de la Drosophile son hôte. Ann. Génét. 5, 1–64 (1963).

    Google Scholar 

  • Plus, N.: Etude de la multiplication du virus de la sensibilité au gaz carbonique chez la Drosophile. Bull. biol. France Belg. 88, 248–293 (1954).

    Google Scholar 

  • Pringle, C. R., Duncan, I. B.: Preliminary physiological characterization of temperaturesensitive mutants of vesicular stomatitis virus. J. Virol. 8, 56–61 (1971).

    Google Scholar 

  • Printz, P.: Influence de facteurs inhibant les synthèses sur le rendement final en virions de Drosophiles propageant le virus σ à l'état stabilisé. C.R. Acad. Sci. (Paris) 258, 378–381 (1964).

    Google Scholar 

  • Printz, P.: Adaptation du virus de la stomatite vésiculaire à Drosophila melanogaster. Ann. Inst. Pasteur 119, 520–537 (1970).

    Google Scholar 

  • Sakuma, S., Watanabe, Y.: Unilateral synthesis of reovirus double-stranded ribonucleic acid by a cell-free replicase system. J. Virol. 8, 190–196 (1971).

    Google Scholar 

  • Watanabe, Y., Gauntt, C. J., Graham, A. F.: Reovirus-induced ribonucleic acid polymerase. J. Virol. 2, 869–877 (1968).

    Google Scholar 

  • Wong, P. K. Y., Holloway, A. F., Cormack, D. V.: A search for recombination between temperature-sensitive mutants of vesicular stomatitis virus. J. gen. Virol. 13, 477–479 (1971).

    Google Scholar 

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  1. Laboratoire de Génétique des Virus, C.N.R.S., Gif-sur-Yvette

    Didier Contamine

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  1. Didier Contamine
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Contamine, D. Étude de mutants thermosensibles du virus Sigma. Molec. Gen. Genet. 124, 233–246 (1973). https://doi.org/10.1007/BF00293094

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  • DOI: https://doi.org/10.1007/BF00293094

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