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Mitochondrial genetics

IV. Allelism and mapping studies of oligomycin resistant mutants in S. cerevisiae

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Summary

  1. 1.

    Two loci O Iand O IIlocalized on the mitochondrial DNA and conferring oligomycin resistance in S. cerevisiae have been demonstrated. The two loci show a high frequency of recombination with each other.

  2. 2.

    In the majority of OR strains examined the mutation was found to map at the O Ilocus.

  3. 3.

    Mapping studies have indicated that the O Iand O IIloci are either unlinked or very weakly linked to each other. Both of these loci also appear to be essentially unlinked from the ω-R I-R II-R IIIsegment of the mitochondrial genome specifying mitoribosomal functions.

  4. 4.

    Analysis of crosses involving mutants at either the O Ior O IIloci and a series of ϱ− petites variously deleted in known mitochondrial genes has demonstrated that the two oligomycin resistance loci O Iand O IIare separable. Assuming that the non deleted segment of the mitochondrial DNA is continuous, the results suggest the gene order ω-R I-R II-R III-O I-O II.

  5. 5.

    The rules applicable to the system and previously delineated from studies of the R I, R IIand R IIIloci in oligomycin sensitive strains have been shown to apply equally to strains carrying OR alleles at the O Iand O IIloci. Oligomycin resistance alleles at both the O loci, O Iand O II, are present in both ω+ and ω− strains.

  6. 6.

    The presence of a nuclear gene(s) in a strain D6, influencing certain facets of the recombination process in both homosexual and heterosexual crosses has been demonstrated and this behaviour is superimposed upon, and clearly separable from the effects due to the mitochondrial gene ω.

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Abbreviations

CS/CR :

Allelic forms of the R Ilocus conferring chloramphenicol sensitivity/resistance

ES/ER :

Allelic forms of either the R IIor R IIIloci conferring erythromycin sensitivity/resistance

OS/OR :

Allelic forms of either of the loci conferring oligomycin sensitivity/resistance

O Iand O II :

Non allelic mitochondrial loci conferring oligomycin resistance

R I :

The mitochondrial locus conferring chloramphenicol resistance

R IIand R III :

Non allelic mitochondrial loci conferring erythromycin resistance

ω+/ω− :

Allelic forms of the mitochondrial sex factor

ϱ+ :

“grande” or respiratory competent cells

ϱ− :

“petite” or cytoplasmically inherited respiratory deficient cells

C0, E0, O0 :

Deletions for the antibiotic resistance genes C, E and O

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Authors and Affiliations

  1. Department of Molecular Sciences, University of Warwick, Coventry, Warwickshire, England

    P. R. Avner

  2. Centre de Génétique Moléculaire du Centre National de la Recherche Scientifique, Gif sur Yvette, France

    D. Coen, B. Dujon & P. P. Slonimski

Authors
  1. P. R. Avner
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  2. D. Coen
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  3. B. Dujon
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  4. P. P. Slonimski
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Communicated by W. Gajewski

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Avner, P.R., Coen, D., Dujon, B. et al. Mitochondrial genetics. Molec. Gen. Genet. 125, 9–52 (1973). https://doi.org/10.1007/BF00292982

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