Skip to main content
SpringerLink
Log in

Labelling of DNA and differential sister chromatid staining after BrdU treatment in vivo

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

A method of labelling DNA in vivo with 5-bromodeoxyuridine (BrdU) is described. After 6 h permanent subcutaneous infusion of BrdU in rodents (adult Microtus agrestis, pregnant NMRI-mice), cell nuclei which have undergone DNA synthesis during the BrdU treatment can be differentiated from the nuclei of other cycle stages by means of their altered staining behaviour after Giemsa. 24 h after the BrdU treatment, mitoses from both bone marrow of the adult animals and tissues from the fetuses showed a differential sister chromatid staining. In male M. agrestis, sister chromatid exchanges were most frequently found in the euchromatic part of the X and in the constitutive heterochromatin of both sex chromosomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bloom, S.E., Hsu, T.C.: Differential fluorescence of sister chromatids in chicken embryos exposed to 5-bromodeoxyuridine. Chromosoma (Berl.) 51, 261–267 (1975)

    Google Scholar 

  • Dutrillaux, B., Fosse, A.M., Prieur, M., Lejeune, J.: Analyse des échanges de chromatides dans les cellules somatiques humaines. Traitement au BrdU (5 bromodéoxyuridine) et fluorescence bicolore par l'Acridine Orange. Chromosoma (Berl.) 48, 327–340 (1974)

    Google Scholar 

  • Kim, M.A.: Chromatidaustausch und Heterochromatinveränderungen menschlicher Chromosomen nach BrdU-Markierung. Humangenetik 25, 179–188 (1974)

    Google Scholar 

  • Latt, S.A.: Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc. nat. Acad. Sci. (Wash.) 70, 3395–3399 (1973)

    Google Scholar 

  • Latt, S.A.: Detection of DNA synthesis in interphase nuclei by fluorescence microscopy. J. Cell Biol. 62, 546–550 (1974)

    Google Scholar 

  • Perry, P., Wolff, S.: New Giemsa method for the differential staining of sister chromatids. Nature (Lond.) 251, 156–158 (1974)

    Google Scholar 

  • Wolf, U., Flinspach, G., Böhm, R., Ohno, S.: DNS-Reduplikationsmuster bei den Riesen-Geschlechtschromosomen von Microtus agrestis. Chromosoma (Berl.) 16, 609–617 (1965)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Anatomisches Institut der Universität Bonn, Nußallee 10, D-5300, Bonn, BRD

    F. Pera & P. Mattias

Authors
  1. F. Pera
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Mattias
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pera, F., Mattias, P. Labelling of DNA and differential sister chromatid staining after BrdU treatment in vivo. Chromosoma 57, 13–18 (1976). https://doi.org/10.1007/BF00292946

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00292946

Keywords

Search

Navigation