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Chromosoma

, Volume 57, Issue 2, pp 199–204 | Cite as

Chromosomal localization of ribosomal 5 S RNA genes in Chironomus thummi by in situ hybridization of iodinated 5S RNA

  • Helmut Bäumlein
  • Ulrich Wobus
Article

Abstract

5 S RNA of Chironomus thummi larvae was purified from total phenol extracted RNA by gel filtration and labelled to about 107 dpm/μg with carrier-free iodine-125. After hybridization in situ of 125I-5S RNA and autoradiography only region B3c-e (containing two “normal” and two very faint bands) of chromosome II of salivary gland cells was highly labelled. In chromosomes of an animal showing pairing discontinuities a clearly “heterozygous” labelling of the 5 S RNA region was found. Region B3c-e shows no clearcut morphological signs of puffing or autoradiographically detectable 3H-uridine incorporation in spite of a continuous synthesis of 5 S RNA in salivary gland cells.

Keywords

Phenol Salivary Gland Developmental Biology Chromosomal Localization Total Phenol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Helmut Bäumlein
    • 1
  • Ulrich Wobus
    • 1
  1. 1.Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDRGaterslebenGerman Democratic Republic

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