, Volume 57, Issue 2, pp 199–204 | Cite as

Chromosomal localization of ribosomal 5 S RNA genes in Chironomus thummi by in situ hybridization of iodinated 5S RNA

  • Helmut Bäumlein
  • Ulrich Wobus


5 S RNA of Chironomus thummi larvae was purified from total phenol extracted RNA by gel filtration and labelled to about 107 dpm/μg with carrier-free iodine-125. After hybridization in situ of 125I-5S RNA and autoradiography only region B3c-e (containing two “normal” and two very faint bands) of chromosome II of salivary gland cells was highly labelled. In chromosomes of an animal showing pairing discontinuities a clearly “heterozygous” labelling of the 5 S RNA region was found. Region B3c-e shows no clearcut morphological signs of puffing or autoradiographically detectable 3H-uridine incorporation in spite of a continuous synthesis of 5 S RNA in salivary gland cells.


Phenol Salivary Gland Developmental Biology Chromosomal Localization Total Phenol 
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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Helmut Bäumlein
    • 1
  • Ulrich Wobus
    • 1
  1. 1.Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDRGaterslebenGerman Democratic Republic

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