Abstract
Ribosomal genes have been localized on mitotic and lampbrush chromosomes of 20 specimens of Triturus vulgaris meridionalis by in situ hybridization with 3H 18S+28S rRNA. The results may be summarized as follows: 1) each individual shows positive in situ hybridization at the nucleolus organizing region (NOR) on chromosome XI; 2) in addition, many specimens exhibit a positive reaction in chromosomal sites other than the NOR (additional ribosomal sites); 3) the chromosomal distribution of the additional sites appears to be identical in different tissues from the same specimen and to follow a specific individual pattern; 4) the additional ribosomal sites are preferentially found at the telomeric, centromeric or C-band regions of the chromosomes involved.
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Abbreviations
- rRNA:
-
ribosomal RNA
- NOR:
-
nucleolus organizer region
- rDNA:
-
the DNA sequences coding for 18S+28S rRNA plus the intervening spacer sequences
- SSC:
-
0.15 M sodium chloride, 0.015 sodium citrate, pH 7
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Nardi, I., Barsacchi-Pilone, G., Batistoni, R. et al. Chromosome location of the ribosomal RNA genes in Triturus vulgaris meridionalis (Amphibia, Urodela). Chromosoma 64, 67–84 (1977). https://doi.org/10.1007/BF00292889
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DOI: https://doi.org/10.1007/BF00292889