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The genetic control of nucleolus formation in wheat

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Abstract

The wheat variety Chinese Spring has four pairs of nucleolus organisers of known rDNA content. The genetic control of these has been investigated in root tip cells by cytologically scoring the number of nucleoli per cell in (a) aneuploid derivatives each having a different dosage of a particular chromosome or chromosome arm and (b) in substitution lines where nucleolus organiser chromosomes have been replaced by homologues possessing different amounts of rDNA. It has been assumed that nucleolus organiser activity is correlated with nucleolus size and thus with the presence of a cytologically visible nucleolus. Those nucleolus organisers on chromosomes 1A and 5D, which together possess only 10% of the rDNA form a visible nucleolus only infrequently in the presence of the larger nucleolus organisers on chromosomes 1B and 6B. When a major pair of organisers on chromosomes 1B or 6B is deleted, the smaller nucleolus organisers form a visible nucleolus more frequently. Similarly, when the major nucleolus organisers are replaced by organisers with less rDNA, the smaller nucleolus organisers form visible nucleoli more frequently. When a small nucleolus organiser is replaced by one with much more rDNA, a larger nucleolus is formed. These and other findings lead to the general conclusions that there is a frequently, but not invariably, seen correlation between rRNA gene number and nucleolus size. However the relative size of the nucleolus formed depends principally upon the proportion of the total active rRNA genes in the cell which are localised at the nucleolus organiser in question. Varying the dosage of at least 13 non nucleolus organiser chromosomes also resulted in changes in the number of visible nucleoli per cell. This implies the genetic control of individual nucleolus organisers is complex. Inclusion in the wheat genome of the nucleolus organiser chromosome from Aegilops umbellulata, causes suppression of the wheat nucleolus organisers, the Aegilops umbellulata organiser remaining active. This suppression is similar to that observed in many interspecific plant and animal hybrids.

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References

  • Bhowal, J.G.: Nucleolar chromosomes in wheat. Z. Pflanzenzüchtung 68, 253–257 (1972)

    Google Scholar 

  • Birnstiel, M.L., Chipchase, M., Spiers, J.: The ribosomal RNA cistrons. Prog. Nucl. Acid Res. molec. Biol. 11, 351–389 (1971)

    Google Scholar 

  • Bramwell, M.E., Handmaker, S.D.: Ribosomal RNA synthesis in human-mouse hybrid cells. Biochim. biophys Acta (Amst.) 232, 580–583 (1971)

    Google Scholar 

  • Croce, C., Talavera, A.: Suppression of production of mouse 28S rRNA in mouse human hybrids segregating mouse chromosomes. Proc. nat. Acad. Sci. (Wash.) 74, 694–697 (1977)

    Google Scholar 

  • Crosby, A.R.: Nucleolar activity of lagging chromosomes in wheat. Amer. J. Bot. 44, 813–822 (1957)

    Google Scholar 

  • Cullis, C.A., Davies, D.R.: Ribosomal DNA amounts in Pisum sativum. Genetics 81, 485–492 (1975)

    Google Scholar 

  • Darvey, N.L., Driscoll, C.J.: Nucleolar behaviour in Triticum. Chromosoma (Berl.) 36, 131–139 (1972)

    Google Scholar 

  • Doerschug, E.B.: Placement of genes for ribosomal RNA within the nucleolar organiser body of Zea mays. Chromosoma (Berl.) 55, 43–56 (1976)

    Google Scholar 

  • Eliceiri, G.L., Green, H.: Ribosomal RNA synthesis in human-mouse hybrid cells. J. molec. Biol. 41, 253–260 (1969)

    Google Scholar 

  • Flavell, R.B., Barratt, D.H.P.: Mitochondrial efficiency and grain yield in wheat. Ann. Bot. 41, 1323–1331 (1977)

    Google Scholar 

  • Flavell, R.B., McPherson, F.J.: The control of wheat malate dehydrogenase by rye chromosomes. Biochem. Genet. 7, 259–268 (1972)

    Google Scholar 

  • Flavell, R.B., O'Dell, M.: Ribosomal RNA genes on homoeologous chromosomes of Groups 5 and 6 in hexaploid wheat. Heredity 37, 377–385 (1976)

    Google Scholar 

  • Flavell, R.B., Smith, D.B.: The role of homoeologous Group 1 chromosomes in the control of rRNA genes in wheat. Biochem. Genetics 12, 271–279 (1974a)

    Google Scholar 

  • Flavell, R.B., Smith, D.B.: Variation in nucleolar organiser rRNA gene multiplicity in wheat and rye. Chromosoma (Berl.) 47, 327–334 (1974b)

    Google Scholar 

  • Givens, J.F., Phillips, R.L.: The Nucleolus organiser region of maize (Zea mays L.). Chromosoma (Berl.) 57, 103–117 (1976)

    Google Scholar 

  • Honjo, T., Reeder, R.H.: Preferential transcription of Xenopus laevis ribosomal RNA in interspecies hybrids between Xenopus laevis and Xenopus mulleri. J. molec. Biol. 80, 217–228 (1973)

    Google Scholar 

  • Ingle, J., Timmis, J.N., Sinclair, J.: The relationship between satellite deoxyribonucleic acid, ribosomal ribonucleic acid gene redundancy and genome size in plants. Plant Physiol. 55, 496–501 (1975)

    Google Scholar 

  • Kasha, K.J., Sadasivaiah, R.S.: Genome relationships between Hordeum vulgare L. and H. bulbosum L. Chromosoma (Berl.) 35, 264–287 (1971)

    Google Scholar 

  • Keep, E.: Satellite and nucleolar number in hybrids between Ribes nigrum and R. grosularia and in their backcrosses. Canad. J. Genet. Cytol. 4, 206–218 (1962)

    Google Scholar 

  • Knowland, J., Miller, L.: Reduction of ribosomal RNA synthesis and ribosomal RNA genes in a mutant of Xenopus laevis which organises only a partial nucleolus. I. Ribosomal RNA synthesis in embryos of different nucleolar types. J. molec. Biol. 53, 321–328 (1970)

    Google Scholar 

  • Krider, H., Plaut, W.: Studies on nucleolar RNA synthesis in Drosophila melanogaster. I. J. Cell Sci. 11, 675–687 (1972a)

    Google Scholar 

  • Krider, H., Plaut, W.: Studies on nucleolar RNA synthesis in Drosophila melanogaster. IL J. Cell Sci. 11, 689–697 (1972b)

    Google Scholar 

  • Law, C.N.: The Development and use of intervarietal chromosome substitution. Europ. Wheat Aneuploid Coop. Newsl. 1, 22–33 (1968)

    Google Scholar 

  • Law, C.N., Worland, A.J.: Aneuploidy in wheat and its uses in genetic analysis. Ann. Rep. Plant Breeding Institute, Cambridge, pp. 25–65 (1972)

    Google Scholar 

  • Longwell, A.C., Svihla, G.: Specific chromosomal control of the nucleolus and cytoplasm in wheat. Exp. Cell Res. 20, 294–312 (1960)

    Google Scholar 

  • Macgregor, H.C., Vlad, M., Barnelt, L.: An investigation of some problems concerning nucleolus organisers in salamanders. Chromosoma (Berl.) 59, 283–299

  • McClintock, B.: The relation of a particular chromosomal element to the development of the nucleoli in Zea mays. Z. Zellforsch. 21, 294–324 (1934)

    Google Scholar 

  • Miller, L., Brown, D.D.: Variation in the activity of nucleolus organisers and their ribosomal gene content. Chromosoma (Berl.) 28, 430–444 (1969)

    Google Scholar 

  • Miller, L., Gurdon, J.B.: Mutations affecting the size of the nucleolus in Xenopus laevis. Nature (Lond.) 227, 1108–1110 (1970)

    Google Scholar 

  • Miller, L., Knowland, J.: Reduction of Ribosomal RNA synthesis and ribosomal RNA genes in a mutant of Xenopus laevis which organises on a partial nucleolus. II. The number of ribosomal RNA genes in animals of different nucleolar types. J. molec. Biol. 53, 329–338 (1970)

    Google Scholar 

  • Miller, O.J., Miller, D.A.: Expression of human and suppression of mouse nucleolus organiser activity in mouse-human somatic cell hybrids. Proc. nat. Acad. Sci. (Wash.) 12, 4531–4535 (1976)

    Google Scholar 

  • Mohan, J., Flavell, R.B.: Ribosomal RNA genes and nucleolar organisers in hexaploid wheat. Genetics 76, 33–44 (1974)

    Google Scholar 

  • Phillips, R.L.: Molecular Cytogenetics of the nucleolus organiser region. In: Genetics and breeding if maize (D.B. Waiden, ed.). London: John Wiley & Sons 1978

    Google Scholar 

  • Phillips, R.L., Kleese, R.A., Wang, S.S.: The nucleolus organiser region of maize (Zea mays L.). Chromosomal site of DNA complementary to ribosomal RNA. Chromosoma (Berl.) 36, 79–88 (1971)

    Google Scholar 

  • Ramirez, S.A., Sinclair, J.H.: Intraspecific variation of ribosomal gene redundancy in Zea mays. Chromosoma (Berl.) 80, 495–504 (1975a)

    Google Scholar 

  • Ramirez, S.A., Sinclair, J.H.: Ribosomal gene localisation and distribution (arrangement) within the nucleolar organiser region of Zea mays. Genetics 80, 505–518 (1975b)

    Google Scholar 

  • Scheer, U., Trendelenburg, M.F., Franke, W.W.: Regulation of transcription of genes of ribosomal RNA during amphibian oogenesis. J. Cell Biol. 69, 465–489 (1976)

    Google Scholar 

  • Sears, E.R.: The aneuploids of common wheat. Missouri Agricultural Exp. Stat. Res. Bull. 572, 1–59 (1954)

    Google Scholar 

  • Shermoen, A.W., Kiefer, B.I.: Regulation in rDNA deficient Drosophila melanogaster. Cell 4, 275–280 (1975)

    Google Scholar 

  • Sinclair, J.H., Carroll, C.R., Humphrey, R.R.: Variation in rDNA redundancy level and NOR length in normal and variant lines of the Mexican axolotyl. J. Cell Sci. 15, 239–258 (1974)

    Google Scholar 

  • Timmis, J., Ingle, J.: Quantitative Regulation of gene activity in plants. Plant Physiol. 56, 255–258 (1975)

    Google Scholar 

  • Timmis, J.N., Sinclair, J., Ingle, J.: Ribosomal RNA genes in euploids and aneuploids of hyacinth. Cell Differentiation 1, 335–339 (1972)

    Google Scholar 

  • Viegas, W., Mello-Sampayo, J.: Nucleolar Organisation in the genus Triticum. Brotéria (Ciencias Naturais) 44, 121–133 (1975)

    Google Scholar 

  • Wallace, H., Langridge, W.H.R.: Differential amphiplasty and the control of ribosomal RNA synthesis. Heredity 27, 1–13 (1971)

    Google Scholar 

  • Weber, D.F.: Nullisomic analysis of Nucleolar formation in Zea mays. Canad. J. Genet. Cytol. 20, 97–100 (1978)

    Google Scholar 

  • Wilkinson, J.: The cytology of Salix in relation to its taxonomy. Ann. Bot. 8, 269–284 (1944)

    Google Scholar 

  • Yeh, B.P., Peloquin, S.J.: The nucleolus associated chromosome of Solanum species and hybrids. Amer. J. Bot. 52, 626 (1965)

    Google Scholar 

Download references

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Authors and Affiliations

  1. Cytogenetics Department, Plant Breeding Institute, CB2 2LQ, Trumpington, Cambridge, England

    Richard B. Flavell & Michael O'Dell

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  1. Richard B. Flavell
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  2. Michael O'Dell
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Flavell, R.B., O'Dell, M. The genetic control of nucleolus formation in wheat. Chromosoma 71, 135–152 (1979). https://doi.org/10.1007/BF00292819

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  • DOI: https://doi.org/10.1007/BF00292819

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