Abstract
Analysis of the organization of nucleotide sequences in mouse genome is carried out on total DNA at different fragment size, reannealed to intermediate value of Cot, by Ag+-Cs2SO4 density gradient centrifugation. — According to nuclease S-1 resistance and kinetic renaturation curves mouse genome appears to be made up of non-repetitive DNA (76% of total DNA), middle repetitive DNA (average repetition frequency 2×104 copies, 15% of total DNA), highly repetitive DNA (8% of total DNA) and fold-back DNA (renatured density 1.701 g/ml, 1% of total DNA).— Non-repetitive sequences are intercalated with short middle repetitive sequences. One third of non-repetitive sequences is longer than 4500 nucleotides, another third is long between 1800 and 4500 nucleotides, and the remainder is shorter than 1800 nucleotides. —Middle repetitive sequences are transcribed in vivo. The majority of the transcribed repeated sequences appears to be not linked to the bulk of non-repeated sequences at a DNA size of 1800 nucleotides. — The organization of mouse genome analyzed by Ag+-Cs2SO4 density gradient of reannealed DNA appears to be substantially different than that previously observed in human genome using the same technique.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Biro, P.A., Carr-Brown, A., Southern, E.M., Walker, P.M.: Partial sequence analysis of mouse satellite DNA: evidence for short range periodicities. J. molec. Biol. 94, 71–86 (1975)
Britten, R.J., Graham, D.E., Neufeld, B.R.: Analysis of repeating DNA sequences by reassociation. In: Methods in enzymology (L. Grossman and K. Moldave, eds.), Vol. 29, Part E, pp. 363–418. New York: Academic Press 1974
Britten, R.J., Kohne, D.E.: Repeated sequences in DNA. Science 161, 529–540 (1968)
Cech, T.R., Hearst, J.E.: Organization of highly repeated sequences in mouse main-band DNA. J. molec. Biol. 100, 227–256 (1976)
Cech, T.R., Rosenfeld, A., Hearst, J.E.: Characterization of the most rapidly renaturing sequences in mouse main-band DNA. J. molec. Biol. 81, 299–325 (1973)
Corneo, G., Ginelli, E., Polli, E.: Repeated sequences in human DNA. J. molec. Biol. 48, 319–327 (1970)
Corneo, G., Ginelli, E., Soave, C, Bernardi, C.: Isolation and characterization of mouse and guinea pig satellite DNA. Biochemistry 7, 4373–4379 (1968)
Crain, W.R., Eden, F.C., Pearson, W.R., Davidson, E.H., Britten, R.J.: Absence of short period interspersion of repetitive and non-repetitive sequences in the DNA of Drosophila melanogaster. Chromosoma (Berl.) 56, 309–326 (1976)
Davidson, E.H., Galau, G.A., Angerer, R.C., Britten, R.J.: Comparative aspects of DNA organization in metazoa. Chromosoma (Berl.) 51, 253–259 (1975)
Davidson, E.H., Hough, B.R., Amenson, C.S., Britten, R.J.: General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus. J. molec. Biol. 77, 1–23 (1973)
Flamm, W.G., Walker, P.M.B., McCallum, M.: Some properties of the single strands isolated from the DNA of the nuclear satellite of the mouse (Mus musculus). J. molec. Biol. 40, 423–443 (1969)
Ginelli, E., Corneo, G.: The organization of repeated DNA sequences in the human genome. Chromosoma (Berl.) 56, 55–68 (1976)
Jensen, R.H., Davidson, N.: Spectrophotometric, potentiometric, and density gradient ultracentrifugation studies of the binding of silver ions by DNA. Biopolymers 4, 17–32 (1966)
Laird, C.D., McConaughy, B.L., McCarthy, B.J.: Rate of fixation of nucleotide substitutions in evolution. Nature (Lond.) 224, 149–154 (1969)
Lewin, B.: Units of transcription and translation: sequence components of heterogeneous nuclear RNA and messenger RNA. Cell 4, 77–93 (1975)
Manning, J.E., Schmid, C., Davidson, N.: Interspersion of repetitive and non-repetitive DNA sequences in the Drosophila melanogaster genome. Cell 4, 141–155 (1975)
Marmur, J.: A procedure for the isolation of deoxyribonucleic acids from micro-organism. J. molec. Biol. 3, 208–218 (1961)
Marmur, J., Doty, P.: Determination of the base composition of deoxyribonucleic acids from its thermal denaturation temperature. J. molec. Biol. 5, 109–118 (1962)
Melli, M., Ginelli, E., Corneo, G., Di Lernia, R.: Clustering of DNA sequences complementary to repetitive nuclear RNA of HeLa cells. J. molec. Biol. 93, 23–38 (1975)
Pagulatos, G.N., Darnell, J.E.: Fractionation of heterogeneous nuclear RNA: rates of hybridization and chromosomal distribution of reiterated sequences. J. molec. Biol. 54, 517–535 (1970)
Southern, E.M.: Long range periodicities in mouse satellite DNA. J. molec. Biol. 94, 51–69 (1975)
Studier, F.W.: Sedimentation studies of the size and shape of DNA. J. molec. Biol. 11, 373–390 (1965)
Sutton, W.D.: A crude nuclease preparation suitable for use in DNA reassociation experiments. Biochim. biophys. Acta (Amst.) 240, 522–531 (1971)
Sutton, W.D., McCallum, M.: Mismatching and the reassociation rate of mouse satellite DNA. Nature (Lond.) New Biol. 232, 83–85 (1971)
Ullman, J.S., McCarthy, B.J.: The relationship between mismatched base pairs and thermal stability of DNA duplexes. I. Effects of depurination and chain scission. Biochim. biophys. Acta (Amst.) 294, 405–424 (1973)
Wilson, D.A., Thomas, C.A. Jr.: Palindromes in chromosomes. J. molec. Biol. 84, 115–144 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ginelli, E., Di Lernia, R. & Corneo, G. The organization of DNA sequences in the mouse genome. Chromosoma 61, 215–226 (1977). https://doi.org/10.1007/BF00292806
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00292806