Abstract
An analysis of the polypeptides synthesized in the salivary glands of late fourth instar R. americana larvae was accomplished by the use of electrophoresis followed by fluorography of in vitro labelled proteins. It was possible to detect five polypeptides which are synthesized only when the giant DNA puffs occur. One of these polypeptides was tentatively assigned to DNA puff B2 and another to the DNA puff C3. This assignment was based on correlations of polypeptide labelling, puffing patterns and RNA synthesizing capacity of the puffs in different sections of the gland during development. The possible meanings and implications of these DNA puffs are discussed.
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Winter, C.E., de Blanchi, A.G., Terra, W.R. et al. Relationships between newly synthesized proteins and DNA puff patterns in salivary glands of Rhynchosciara americana . Chromosoma 61, 193–206 (1977). https://doi.org/10.1007/BF00292804
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DOI: https://doi.org/10.1007/BF00292804