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Polytene chromosomes from ovarian pseudonurse cells of the Drosophila melanogaster otu mutant

I. Photographic map of chromosome 3

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Abstract

Certain mutant alleles of the otu locus in Drosophila melanogaster produce abnormal nurse cells in the ovaries. These cells are called pseudonurse cells (PNC), since they generate polytene chromosomes instead of endopolyploid ones and do not normally have an oocyte to nurse. The banding pattern of polytene chromosome 3 from the salivary glands (SG) and from PNCs of homozygous otu 1 females was compared and a detailed photomap of PNC chromosomes with different degrees of polyteny is presented. The banding pattern was found to be strikingly similiar in the two tissues. The puffing pattern of the PNC chromosomes was also studied and the function of the PNC chromosomes is discussed. No constrictions or breaks were found in the PNC chromosomes which seems to indicate that these sites, which are known to be underreplicated in the SG chromosomes, are equally replicated along with the rest of the chromosomes in the PNC nuclei.

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  1. Department of Genetics, University of Helsinki, Arkadiankatu 7, SF-00100, Helsinki, Finland

    Tapio I. Heino

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Heino, T.I. Polytene chromosomes from ovarian pseudonurse cells of the Drosophila melanogaster otu mutant. Chromosoma 97, 363–373 (1989). https://doi.org/10.1007/BF00292763

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  • DOI: https://doi.org/10.1007/BF00292763

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