Abstract
Surface-spread and silver-stained spermatocytes of Peromyscus maniculatus and P. sitkensis were analyzed in order to develop criteria for the recognition of meiotic substages from early zygonema through early diplonema. The continuous sequence of changes in the morphology of the autosomal axes (lateral elements) of the synaptonemal complexes (SC), sex chromosome axes, and nucleoli enabled the recognition of three substages of zygonema and five of pachynema. The proposed system of subdivision is compatible with descriptions of comparable data from Chinese hamsters and laboratory mice with differences being primarily associated with the timing of sex chromosome synapsis and desynapsis. Within the substages, cytogenetically important details of the synaptic mechanism in deer mice were noted. Autosomal synaptic initiation in deer mice is apparently uniterminal, involving the distal (noncentromeric) end of the homologs. Subsequent pairing is unidirectional towards the centromeric end. Additionally, during mid and late zygonema the homologous axes of late pairing regions of some autosomes were characterized by substantial length differences. These lateral element length differences were not maintained into pachynema and it is hypothesized that differences in the amount of material in the heterochromatic short arms of these species may be subject to synaptic adjustment.
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References
Ashley T (1983) Nonhomologous synapsis of the sex chromosomes in the heteromorphic bivalents of two X-7 translocations in male mice: R5 and R6. Chromosoma 88:178–183
Ashley T, Moses MJ, Solari AJ (1981) Fine structure and behaviour of a pericentric inversion in the sand rat, Psammomysobesus. J Cell Sci 50:105–119
Ashley T, Russell LB, Cacheiro NLA (1982) Synaptonemal complex analysis of two X-7 translocations in male mice: R3 and R5 quadrivalents. Chromosoma 87:149–164
Ashley T, Russell LB, Cacheiro NLA (1983) Synaptonemal complex analysis of two X-7 translocations in male mice: R2 and R6 quadrivalents. Chromosoma 88:171–177
Committee for the standardization of chromosomes of Peromyscus (1977) Standardized karyotype of the deer mouse, Peromyscus (Rodentia). Cytogenet Cell Genet 19:38–43
Counce SJ, Meyer GF (1973) Differentiation of the Synaptonemal complex and the kinetochore in Locusta spermatocytes studied by whole mount electron microscopy. Chromosoma 44:231–253
Davisson MT, Poorman PA, Roderick TH, Moses MJ (1981) A pericentric inversion in the mouse. Cytogenet Cell Genet 30:70–76
Dresser ME, Moses MJ (1979) Silver staining of Synaptonemal complexes in surface spreads for light and electron microscopy. Exp Cell Res 121:416–419
Dresser ME, Moses MJ (1980) Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus) IV. Light and electron microscopy of synapsis and nucleolar development by silver staining. Chromosoma 76:1–22
Fletcher JM (1979) Light microscopic analysis of meiotic prophase chromosomes by silver staining. Chromosoma 72:241–248
Goldstein P, Moens PB (1976) Karyotype analysis of Ascaris lumbricoides var. suum. Male and female pachytene nuclei by 3-D reconstruction from electron microscopy of serial sections. Chromosoma 58:101–111
Goldstein P, Slaton DE (1982) Synaptonemal complexes of Caenorhabditis elegans. Comparison of wild type and mutant strains and pachytene karyotype analysis of wild type. Chromosoma 84:585–597
Goldstein P, Triantaphyllou AC (1978) Occurrence of Synaptonemal complexes and recombination nodules in a meiotic race of Meloidogyne hapla and their absence in a mitotic race. Chromosoma 68:91–100
Greenbaum IF, Baker RJ (1978) Determination of the primitive karyotype for Peromyscus. J Mammal 59:820–834
Greenbaum IF, Reed MJ (1984) Evidence for heterosynaptic pairing of the inverted segment in pericentric inversion heterozygotes of the deer mouse (Peromyscus maniculatus). Cytogenet Cell Genet 38:106–111
Greenbaum IF, Baker RJ, Bowers JH (1978) Chromosomal homology and divergence between sibling species of deer mice: Peromyscus maniculatus and P. melanotis (Rodentia, Cricetidae). Evolution 32:334–341
Holm PB, Rasmussen SW (1983) Human meiosis V. Substages of pachytene in human spermatogenesis. Carlsberg Res Commun 48:351–383
Holm PB, Rasmussen SW, von Wettstein D (1982) Ultrastructural characterization of the meiotic prophase. A tool in the assessment of radiation damage in man. Mutat Res 95:45–59
Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36:1014–1015
Lee MR, Elder FFB (1980) Yeast stimulation of bone marrow mitosis for cytogenetic investigations. Cytogenet Cell Genet 26:36–40
Mahadevaiah S, Mittwoch U, Moses MJ (1984) Pachytene chromosomes in male and female mice heterozygous for the Is (7; 1) 40H insertion. Chromosoma 90:163–169
Moses MJ (1977a) Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus) I. Morphology of the autosomal complement in spread preparations. Chromosoma 60:99–125
Moses MJ (1977b) Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus) II. Morphology of the XY pair in spread preparations. Chromosoma 60:127–137
Moses MJ (1980) New cytogenetic studies on mammalian meiosis. In: Serio M, Martini L (eds) Animal models in human reproduction. Raven Press, New York, pp 169–190
Moses MJ, Poorman PA (1981) Synaptonemal complex analysis of mouse chromosomal rearrangements II. Synaptic adjustment in a tandem duplication. Chromosoma 81:519–535
Moses MJ, Poorman PA, Roderick TH, Davisson MT (1982) Synaptonemal complex analysis of mouse chromosomal rearrangements IV. Synapsis and synaptic adjustment in two paracentric inversions. Chromosoma 84:457–474
Moses MJ, Slatton GH, Gambling TM, Starmer CF (1977) Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus) III. Quantitative evaluation. Chromosoma 60:345–375
Pathak S, Hsu TC (1979) Silver-stained structures in mammalian meiotic prophase. Chromosoma 70:195–203
Pengilly D, Jarrell GH, MacDonald SO (1983) Banded karyotypes of Peromyscus sitkensis from Baranof Island, Alaska. J Mammal 64:682–685
Poorman PA, Moses MJ, Davisson MT, Roderick TH (1981a) Synaptonemal complex analysis of mouse chromosomal rearrangements III. Cytogenetic observations on two paracentric inversions. Chromosoma 83:419–429
Poorman PA, Moses MJ, Russell LB, Cacheiro NLA (1981b) Synaptonemal complex analysis of mouse chromosomal rearrangements I. Cytogenetic observations on a tandem duplication. Chromosoma 81:507–518
Quack B, Noel B (1977) The XY chormosome pair in mouse and human spermatocytes visualized by silver-staining. Nature 267:431–433
Rogers DS, Greenbaum IF, Gunn SJ, Engstrom MD (1984) Cytosystematic value of chromosomal inversion data in the genus Peromyscus (Rodentia: Cricetidae). J Mammal 65:457–465
Seabright M (1971) A rapid banding technique for human chromosomes. Lancet 2:971–972
Stangl FB Jr, Baker RJ (1984) Evolutionary relationships in Pero-myscus: congruence in chromosomal, genic, and classical datasets. J Mammal 65:643–654
Sumner AT (1972) A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75:304–306
Takanari H, Pathak S, Hsu TC (1982) Dense bodies in silverstained spermatocytes of the Chinese hamster: Behavior and cytochemical nature. Chromosoma 86:359–373
von Wettstein D, Rasmussen SW, Holm PB (1984) The synaptonemal complex in genetic segregation. Annu Rev Genet 18:331–413
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Greenbaum, I.F., Hale, D.W. & Fuxa, K.P. The mechanism of autosomal synapsis and the substaging of zygonema and pachynema from deer mouse spermatocytes. Chromosoma 93, 203–212 (1986). https://doi.org/10.1007/BF00292739
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DOI: https://doi.org/10.1007/BF00292739