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Development of DNA puffs and patterns of polypeptide synthesis in the salivary glands of Bradysia hygida

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Abstract

In the three salivary gland regions of Bradysia hygida (Diptera, Sciaridae) the patterns of polypeptide synthesis, as revealed by electrophoresis and fluorography, are very stable during the fourth larval instar until about 30 h before the pupal molt. At this age the patterns of polypeptide synthesis start to undergo marked changes. The striking correlations between these changes and the development of two distinct groups of DNA puffs support the proposal that DNA puffs are causally related to the synthesis of specific proteins in the salivary glands.

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References

  • Amabis JM (1980) Biologia dos cromossomos politênicos: seu papel na diferenciação celular. Resumos do II∘ Congresso Brasileiro de Biologia Celular, pp 28–29

  • Bonaldo MF, Santelli RV, Lara FJS (1979) The transcript from a DNA puff of Rhynchosciara and its migration to the cytoplasm. Cell 17:827–833

    Google Scholar 

  • Breuer ME, Pavan C (1955) Behavior of polytene chromosomes of Rhynchosciara angelae at different stages of larval development. Chromosoma 7:371–386

    Google Scholar 

  • Brown DD (1981) Gene expression in eukaryotes. Science 211:667–674

    Google Scholar 

  • De Toledo SM, Lara FJS (1978) Translation of messages transcribed from DNA puffs of Rhynchosciara. Biochem Biophys Res Comm 85:160–166

    Google Scholar 

  • Gabrusewycz-Garcia N (1964) Cytological and autoradiographic studies in Sciara coprophila salivary gland chromosomes. Chromosoma 15:312–344

    Google Scholar 

  • Glover DM, Zaha A, Stocker AJ, Santelli RV, Pueyo MT, De Toledo SM, Lara FJS (1982) Gene amplification in Rynchosciara salivary gland chromosomes. Proc Natl Acad Sci 79:2947–2951

    Google Scholar 

  • Harrison PR, Birnie GD, Hell A, Humphries S, Young BD, Paul J (1974) Kinetic studies of gene frequency. I. Use of a DNA copy of reticulocyte 9 S RNA to estimate globin gene dosage in mouse tissues. J Mol Biol 84:539–554

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685

    Google Scholar 

  • Laskey RA, Mills AD (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem 56:335–341

    Google Scholar 

  • Loening UE (1967) The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel-electrophoresis. Biochem J 102:251–257

    Google Scholar 

  • Packman S, Aviv H, Ross J, Leder P (1972) A comparison of globin genes in duck reticulocytes and liver cells. Biochem Biophys Res Comm 49:813–819

    Google Scholar 

  • Paço-Larson ML (1976) Análise quantitativa do conteúdo de DNA da glândula salivar de Bradysia hygida. Master Thesis, University of São Paulo

  • Rizzo E (1980) Descrição dos pufes cromossômicos da glândula salivar de Bradysia hygida (Diptera, Sciaridae). Masters Thesis, University of São Paulo

  • Sauaia H, Laicine EM (1977) Vertical slab electrophoresis apparatus. Anal Biochem 80:125–132

    Google Scholar 

  • Sauaia H, Laicine EM, Alves MAR (1971) Hydroxyurea-induced inhibition of DNA puff development in the salivary gland chromosomes of Bradysia hygida. Chromosoma 34:129–151

    Google Scholar 

  • Spradling AC, Mahowald P (1980) Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster. Proc Natl Acad Sci 77:1096–1100

    Google Scholar 

  • Sullivan D, Palacios R, Stavnezer J, Taylor JM, Faras AJ, Dily ML, Summers NM, Bishop JM, Schimke RT (1973) Synthesis of a deoxyribonucleic acid sequence complementary to ovalbumin messenger ribonucleic acid and quantitation of ovalbumin genes. J Biol Chem 248:7530–7539

    Google Scholar 

  • Susuki I, Gage LP, Brown DD (1972) The genes for fibroin in Bombyx mori. J Mol Biol 63:409–429

    Google Scholar 

  • Winter CE, de Bianchi AG, Terra WR, Lara FJS (1977) Relationships between newly synthesized proteins and DNA puff patterns in salivary glands of Rhynchosciara americana. Chromosoma 61:193–206

    Google Scholar 

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Laicine, E.M., Alves, M.A.R., de Almeida, J.C. et al. Development of DNA puffs and patterns of polypeptide synthesis in the salivary glands of Bradysia hygida . Chromosoma 89, 280–284 (1984). https://doi.org/10.1007/BF00292475

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  • DOI: https://doi.org/10.1007/BF00292475

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