Abstract
Treatment of Chironomus larvae with dilute (0.5%–1.0%) ethanol results in puffing changes similar to those obtained with galactose in the Balbiani rings (BRs) of the salivary gland chromosomes. A shift in the relative size of BR1 and BR2 in chromosome 4 of C. pallidivittatus or C. tentans was observed within 1–2 days after ethanol treatment. The exceptional Balbiani ring, BR6 in chromosome 3, began to appear within 1 day after ethanol treatment of C. pallidivittatus and was fully developed after 3–4 days. Prepupae appeared to be refractory to the treatment. To localize possible controls of BR puffing in Chironomus, ligatures were made at various positions along the thorax and the anterior abdominal segments of the ethanoltreated larvae. In surviving larvae, ligated anterior to the brain or posterior to the salivary glands, induction of BR6 could be detected. In contrast, little or no BR6 puff induction was found in animals ligated in the middle of the second segment approximately between the brain and the salivary glands. No shift in the BR1/BR2 relation occurred with any of the ligations combined with ethanol treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baudisch W, Panitz R (1968) Kontrolle eines biochemischen Merkmals in den Speicheldrüsen von Acricotopus lucidus durch einen Balbiani-Ring. Exp Cell Res 49:470–476
Beermann W (1952a) Chromomerenkonstanz und spezifische Modifikationen der Chromosomenstruktur in der Entwicklung und Organdifferenzierung von Chironomus tentans. Chromosoma 5:139–198
Beermann W (1952b) Chromosomenstruktur und Zelldifferenzierung in der Speicheldrüse von Trichocladius vitripennsis. Z Naturforsch 7b:237–242
Beermann W (1961) Ein Balbiani-Ring als Locus einer Speicheldrüsenmutation. Chromosoma 12:1–25
Beermann W (1971) Effect of α-amanitine on puffing and intranuclear RNA-synthesis in Chironomus salivary glands. Chromosoma 34:152–167
Beermann W (1973) Directed changes in the pattern of Balbiani ring puffing in Chironomus: Effects of sugar treatment. Chromosoma 41:297–326
Clever U, Karlson P (1960) Induktion von Puff-Veränderungen in den Speicheldrüsenchromosomen von Chironomus tentans durch Ecdyson. Exp Cell Res 20:623–626
Colman OD, Stockert JC (1975) Puffing patterns during the fourth larval instar Chironomus pallidivattatus salivary glands. Chromosoma 53:381–392
Daneholt B (1975) Transcription in polytene chromosomes. Cell 4:1–9
Edström J-E, Sierakowska H, Burvall K (1982) Dependence of Balbiani ring induction in Chironomus salivary glands on inorganic phosphate. Dev Biol 91:131–137
Gronemeyer H, Pongs O (1980) Localization of ecdysterone on polytene chromosomes of Drosophila melanogaster. Proc Nat Acad Sci 77:2108–2112
Grossbach U (1969) Chromosomen-Aktivität und biochemische Zelldifferenzierung in den Speicheldrüsen von Camptochironomus. Chromosoma 28:136–187
Hardy P (1982) The salivary glands of Chironomus tentans: Characterization of the major secretory products and their messenger RNAs. Dissertation, Universität Tübingen
Kroeger H (1963) Chemical nature of the system controlling gene activities in insect cells. Nature 200:1234–1235
Lambert B, Daneholt B, Edström J-E, Egyhazy E, Rinborg U (1973) Comparison between chromosomal and nuclear sap RNA from Chironomus tentans salivary gland cells by RNADNA-hybridization. Exp Cell Res 76:381–389
Pelling C (1964) Ribonukleinsäure-Synthese der Riesenchromosomen. Autoradiographische Untersuchungen an Chironomus tentans. Chromosoma 15:71–122
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yagi, S. Effects of ligation on ethanol-induced Balbiani ring puffing in salivary glands of Chironomus . Chromosoma 89, 274–279 (1984). https://doi.org/10.1007/BF00292474
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00292474