Abstract
A simple method has been described for the visualization of chromosome cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mitotic chromosomes in all the materials employed, confirming the findings of Howell and Hsu (1979). (2) The microchromosomes of the chicken and double minutes of a human carcinoma cell line also exhibited the core structure. (3) The core structure of meiotic chromosomes appear weak, disorganized, and disintegrating.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barker, P.E., Hsu, T.C.: Are double minutes chromosomes? Exp. Cell Res 113, 456–458 (1978)
Dresser, M.E., Moses, M.J.: Silver staining of synaptonemal complexes in surface spreads for light and electron microscopy. Exp. Cell Res. 121, 416–419 (1979)
Dresser, M.E., Moses, M.J.: Synaptonemal complex karyotyping in spermatocytes of the Chinese hamster (Cricetulus griseus). IV. Light and electron microscopy of synapsis and nucleolar development by silver staining. Chromosoma (Berl.) 76, 1–22 (1980)
Fletcher, J.M.: Light microscope analysis of meiotic prophase chromosomes by silver staining. Chromosoma (Berl.) 72, 241–248 (1979)
Forejt, J., Goetz, P.: Synaptonemal complexes of mouse and human pachytene chromosomes visualized by silver staining in air-dried preparations. Chromosoma (Berl.) 73, 255–261 (1979)
Howell, W.M., Hsu, T.C.: Chromosome core revealed by silver staining. Chromosoma (Berl.) 73, 61–66 (1979)
Laemmli, U.K.: Levels of organization of the DNA in eucaryotic chromosomes. Pharmacol. Rev. 30, 469–476 (1979)
Levan, A., Levan, G.: Have double minutes functioning centromeres? Hereditas (Lund) 88, 81–92 (1978)
Pathak, S., Arrighi, F.E.: Loss of DNA following C-banding procedures. Cytogenet. Cell Genet. 12, 414–422 (1973)
Pathak, S., Elder, F.F.B.: Silver stained accessory structures on human sex chromosomes. Hum. Genet. 54, 171–175 (1980)
Pathak, S., Hsu, T.C.: Silver stained structures in mammalian meiotic prophase. Chromosoma (Berl.) 70, 195–203 (1979)
Paulson, J.R., Laemmli, U.K.: The structure of histone-depleted metaphase chromosomes. Cell 12, 817–828 (1977)
Quinn, L.A., Moore, G.E., Morgan, R. T., Woods, L. K.: Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39, 4914–4924 (1979)
Stubblefield, E.: The structure of mammalian chromosomes. Int. Rev. Cytol. 35, 1–60 (1973)
Stubblefield, E., Wray, W.: Architecture of the Chinese hamster metaphase chromosome. Chromosoma (Berl.) 32, 262–294 (1971)
Taylor, J.H.: Duplication of chromosomes. Scientific American 198, 36–42 (1958)
Wray, W., Mace, Jr. M., Daskal, Y., Stubblefield, E.: Metaphase chromosome architecture. Cold Spr. Harb. Symp. quant. Biol. 42, 361–365 (1978)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Satya-Prakash, K.L., Hsu, T.C. & Pathak, S. Behavior of the chromosome core in mitosis and meiosis. Chromosoma 81, 1–8 (1980). https://doi.org/10.1007/BF00292418
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00292418