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Characterization of 65 bovine microsatellites

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Abstract

Microsatellites or simple sequence repeat (SSR) polymorphisms are used widely in the construction of linkage maps in many species. High levels of polymorphism coupled with the ease of analysis of the polymerase chain reaction (PCR) have resulted in this type of maker being one of the most widely used for genetic analysis. In this paper we describe 58 polymorphic bovine microsatellites that were isolated from insert size selected bovine genomic libraries. Primer sequences, number of alleles, and heterozygosity levels in cattle reference families are reported. Chromosomal locations for 47 of these microsatellites as well as for 7 previously described systems derived from entries in the Genbank or EMBL databases have been determined. The markers map to 24 syntenic or chromosomal locations. Polymorphic bovine microsatellites were estimated to occur, on average, every 320 kb, and there is no evidence of clustering in the genome. Thirty of the bovine-derived microsatellite systems gave specific and polymorphic products in sheep, adding to the number of useful markers in that species.

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References

  • Barendse, W., Armitage, S.M., Kossarek, L. M., Shalom, A., Kirkpatrick, B.W., Ryan, A.M., Clayton, D., Li, L., Niebergs, H., Zhang, N., Grosse, M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teale, A.J., Fries, R., McGraw, R.A., Moore, S.S., Georges, M., Soller, M., Womack, J.E., Hetzel, D.J.S. (1993). A genetic linkage map of the bovine genome. Nature Genet., in press.

  • Beckman, J.S., Weber, J.L. (1992). Survey of human and rat microsatellites. Genomics 12, 627–631.

    Google Scholar 

  • Bowcock, A., Osborne-Lawrence, S., Barnes, R., Chakravarti, A., Washington, S., Dunn, C. (1993). Microsatellite polymorphism linkage map of human chromosome 13q. Genomics 15, 376–386.

    Google Scholar 

  • Hediger, R., Johnson, S.E., Barendse, W., Drinkwater, R.D., Moore, S.S., Hetzel, J. (1990). Assignment of the growth hormone gene locus to 19q26-qter in cattle and to 11q25-qter in sheep by in situ hybridisation. Genomics 8, 171–174.

    Google Scholar 

  • Hediger, R., Ansari, H.A., Stranzinger, G.F. (1991a). Chromosome banding and gene localisation support extensive conservation of chromosome structure between cattle and sheep. Cytogenet. Cell Genet 57, 127–134.

    Google Scholar 

  • Hediger, R., Johnson, S.E., Hetzel, D.J.S. (1991b). Localisation of the β subunit of follicle stimulating hormone in cattle and sheep. Anim. Genet. 22, 237–244.

    Google Scholar 

  • Hetzel, D.J.S. (1991). The use of reference families for genome mapping in domestic livestock. In Gene-Mapping Techniques and Applications, L.B. Schook, H.A. Lewin, D.G. McLaren, eds. (New York: Marcel Dekker Inc.), pp. 51–64.

    Google Scholar 

  • Kaukinen, J., Varvio, S.-L. (1992). Artiodactyl retroposons: association with microsatellites and use in SINEmorph detection with PCR. Nucleic Acids Res. 20, 2955–2958.

    Google Scholar 

  • Lange, K., Boehnke, M. (1982) How many polymorphic genes will it take to span the human genome? Am. J. Hum. Genet. 34, 842–845.

    Google Scholar 

  • Litt, M., Luty, J.A. (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am J. Hum. Genet. 4, 397–401.

    Google Scholar 

  • Love, J.M., Knight, A.M., McAleer, M.A., Todd, J.A. (1990). Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites. Nucleic Acids Res. 18, 4123–4130.

    Google Scholar 

  • Maniatis, T., Fitsch, E.E., Sambrook, J. (1982). Molecular Cloning, A Laboratory Manual. (Cold Spring Harbor Laboratory, N.Y.: Cold Spring Harbor Laboratory Press).

    Google Scholar 

  • Moore, S.S., Byrne, K. (1992). Dinucleotide polymorphism at the bovine brain ribonuclease locus. Anim. Genet. 23, 574.

    Google Scholar 

  • Moore, S.S., Byrne, K. (1993a). Dinucleotide polymorphism at the bovine histamine H1 receptor locus. Anim. Genet. 24, 72.

    Google Scholar 

  • Moore, S.S., Byrne, K. (1993b). Dinucleotide polymorphism at the bovine calmodulin independent adenyl cylase locus. Anim. Genet. 24, 150.

    Google Scholar 

  • Moore, S.S., Sargeant L.L., King, T.J., Mattick, J.S., Georges, M., Hetzel, D.J.S. (1991). The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous primer pairs in closely related species. Genomics 10, 654–660.

    Google Scholar 

  • Moore, S.S., Barendse, W., Berger, K.T., Armitage, S.M., Hetzel, D.J.S., (1992). Bovine and ovine DNA microsatellites from the EMBL and GENBANK databases. Anim. Genet. 23, 463–467.

    Google Scholar 

  • O'Brien, S.J., Womack, J.E., Lyons, L.A., Moore, K.J., Jenkins, N.A., Copeland, N.G. (1993). Anchored reference loci for comparative genome mapping in mammals. Nature Genet. 3, 103–112.

    Google Scholar 

  • Ostrander, E.A., Sprague, G.F. Jr., Rine, J. (1993). Identification and characterisation of dinucleotide repeat (CA)n markers for genetic mapping in dog. Genomics 16, 207–213.

    Google Scholar 

  • Paterson, A.H., Lander, E.S., Hewitt, J.D., Peterson, S., Lincoln, S.E., Tanksley, S.D. (1988). Resolution of quantitative traits into Mendelain factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335, 721–726.

    Google Scholar 

  • Sanger, F., Nicklen, S., Coulson, A.R. (1977). DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463–5467.

    Google Scholar 

  • Schlotterer, C., Amos, B., Tautz, D. (1991). Conservation of polymorphic simple sequence loci in cetacean species. Nature 354, 63–65.

    Google Scholar 

  • Stallings, R.L., Ford, A.F., Nelson, D., Torney, D.C., Hildebrand, C.E., Moyzis, R.K. (1991). Evolution and distribution of (GT)n repetitive sequences in mammalian genomes. Genomics 10, 807–815.

    Google Scholar 

  • Steele, M.R., Georges, M. (1991). Generation of bovine multisite haplotypes using random cosmid clones. Genomics 10, 889–904.

    Google Scholar 

  • Tautz, D. (1989). Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Res. 17, 6463–6471.

    Google Scholar 

  • Weber, J.L. (1990). Informativeness of human (dC-DA)n ·(dG-dT)n polymorphisms. Genomics 7, 524–530.

    Google Scholar 

  • Weber J.L., May, P.E. (1989). Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J. Hum. Genet. 44, 388–396.

    Google Scholar 

  • Wintero, A.K., Fredholm, M., Thomsen, P.D. (1992). Variable (dG-dT)n.(dC-dA)n sequences in the porcine genome. Genomics 12, 281–288.

    Google Scholar 

  • Womack, J.E., Moll, Y.D. (1986). Gene map of the cow: conservation of linkage with mouse and man. J. Hered. 77, 2–7.

    Google Scholar 

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Moore, S.S., Byrne, K., Berger, K.T. et al. Characterization of 65 bovine microsatellites. Mammalian Genome 5, 84–90 (1994). https://doi.org/10.1007/BF00292333

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  • DOI: https://doi.org/10.1007/BF00292333

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