, Volume 70, Issue 1, pp 91–99 | Cite as

Chromosome location of the ribosomal RNA genes in Triturus vulgaris meridionalis (Amphibia, Urodela)

IV. Comparison between in situ hybridization with 3H 18S + 28S rRNA and AS-SAT staining
  • I. Nardi
  • S. De Lucchini
  • G. Barsacchi-Pilone
  • F. Andronico


The mitotic chromosomes of six specimens from Triturus vulgaris meridionalis have been examined by both in situ hybridization with 3H 18S+28S rRNA and AS-SAT staining method. The results of these two sets of experiments can be summarized as follows: 1) in each specimen the NORs and the additional ribosomal sites, which react positively to in situ hybridization with 3H 18S + 28S rRNA, are also stained by silver; 2) other chromosomal regions, which do not hybridize in situ with 3H 18S+28S rRNA, are on the other hand stained by the AS-SAT method. These latter Ag-positive sites show a species-specific pattern of chromosomal distribution.


Developmental Biology Chromosomal Region Chromosome Location Staining Method Mitotic Chromosome 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • I. Nardi
    • 1
  • S. De Lucchini
    • 1
  • G. Barsacchi-Pilone
    • 1
  • F. Andronico
    • 1
  1. 1.Istituto di Istologia ed EmbriologiaPisaItaly

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