Abstract
The ribosomal RNA genes from the sea urchin Lytechinus variegatus have been studied with the electron microscope using the technique of denaturation mapping. A repeating pattern of denatured regions was found with an average repeat length of 3.87±0.24μm. This corresponds to a DNA sequence of approximately 12,000 base pairs with a molecular weight of 8×106 daltons.
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Abbreviations
- rRNA:
-
ribosomal RNA, including 26S and 18S RNA
- Tris:
-
tris(hydroxymethyl)-aminomethane
- EDTA:
-
ethylenediaminetetraacetate
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Wilson, F.E., Blin, N. & Stafford, D.W. A denaturation map of sea urchin ribosomal DNA. Chromosoma 58, 247–253 (1976). https://doi.org/10.1007/BF00292090
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DOI: https://doi.org/10.1007/BF00292090