Summary
The kinetics of replication for early and late replicating X chromosomes in karyotypically normal fibroblasts and lymphocytes was studied using terminal bromodeoxyuridine (BrdU) treatment followed by Hoechst/light/Giemsa staining. Although the order of band appearance differs between the two tissues, the programme (order and interval between band appearances) for early replicating bands (dark R-bands) is identical in the two homologues. This is probably also the case for later replicating bands (dark G-bands) though the criteria for derermining mean band appearance times are less reliable for these bands when terminal BrdU treatment is used. This means that the late X has a delayed start but thereafter proceeds at the same pace as its early counterpart.
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Reddy, K.S., Savage, J.R.K. & Papworth, D.G. Replication kinetics of X chromosomes in fibroblasts and lymphocytes. Hum Genet 79, 44–48 (1988). https://doi.org/10.1007/BF00291708
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DOI: https://doi.org/10.1007/BF00291708