Summary
Variation of DA/DAPI intensity in the Yq12 band was observed in five amniotic cell specimens and one blood specimen from the father of one fetus. Three distinct classes of Yq heterochromatin were identified by distamycin A (DA) treatment of the cell cultures and various staining techniques. The heterochromatin in the Yq11.23 sub-band does not under-condense when exposed to DA, and shows pale fluorescence with quinacrine staining, positive C-banding, and bright fluorescence with DA/DAPI technique. This class of heterochromatin was consistently observed in all specimens studied. The other two classes of heterochromatin are in the Yq12 band. Both show undercondensation when exposed to DA, quinacrine-bright fluorescence, and positive C-banding; howover, one class of heterochromatin shows DA/DAPI-bright fluorescence and the other shows pale fluorescence. The size and banding intensity of the two classes of heterochromatin in Yq12 are variable. These results provide cytological evidence of heterogeneity within the Y heterochromatin region containing AT-rich DNA.
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References
Angell RR, Jacobs PA (1975) Lateral asymmetry in human constitutive heterochromatin. Chromosoma 51: 301–310
Babu A, Macera MJ, Verma RS (1986) Intensity heteromorphisms of human chromosome 15p by DA/DAPI technique. Hum Genet 73: 298–300
Bühler EM (1980) A synopsis of the human Y chromosome. Hum Genet 55: 145–175
Goodfellow F, Darling S, Wolfe J (1985) The human Y chromosome. J Med Genet 22: 329–344
Gosden JR, Mitchell AR, Buckland RA, Clayton RP, Evans JH (1975) The location of four human satellite DNA's on human chromosomes. Exp Cell Res 92: 148–158
Gosden JR, Lawrie SS, Cooke HJ (1981) A cloned repeated DNA sequence in human chromosome heteromorphisms. Cytogenet Cell Genet 29: 32–39
ISCN (1985) An international system for human cytogenetics nomen clature (1985). Birth Defects 21, no 1
Latt SA, Davidson RL, Lin MS, Gerald PS (1974) Lateral asymmetry in the fluorescence of human Y chromosomes stained with 33258 Hoechst. Exp Cell Res 87: 425–429
Latt SA, Juergenss LA, Mathews DJ, Gustashaw KM, Sahar E (1981) Energy-enhanced chromosome banding. An overview. Cancer Genet Cytogenet 1: 187–196
Limon J, Gibas Z, Kaluzewski B, Moruzgala T (1979) Demonstration of two different regions of lateral asymmetry in human Y chromosomes. Hum Genet 51: 247–252
Lin MS, Latt SA, Davidson RL (1974) Microfluorometric detection of asymmetry in the centromeric region of mouse chromosomes. Exp Cell Res 86: 392–394
Lin MS, Comings DE, Alfi OS (1977) Optical studies of the interaction of 4′–6-diamidino-2-phenylindole with DNA and metaphase chromosomes. Chromosoma 60: 15–25
Paris Conference 1971 (1972) Standardization in human cytogenetics. Birth Defects 8, no 7
Pérez-Castillo A, Martin-Lucas MA, Abrisqueta JA (1987) Evidence for lack of specificity of the DA/DAPI technique. Cytogenet Cell Genet 45: 62
Prantera G, Pimpinelli S, Rocchi A (1979) Effects of distamycin A on human leukocytes in vitro. Cytogenet Cell Genet 23: 103–107
Rocchi A, Di Cartro M, Prantera G (1979) Effect of DAPI on human leukocytes in vitro. Cytogenet Cell Genet 23: 250–254
Schmid M (1979) Demonstration of Y/autosomal translocations using distamycin A. Hum Genet 53: 107–109
Schnedl W, Mikelsaar AV, Breitenbach M, Dann O (1977) DIPI and DAPI: fluorescence banding with only negligible fading. Hum Genet 36: 167–172
Schweizer D (1980) Simultaneous fluorescent staining of R bands and specific heterochromatic region (DA-DAPI) in human chromosomes. Cytogenet Cell Genet 27: 190–193
Schweizer D, Ambros P, Andrle M (1978) Modification of DAPI banding on human chromosomes with a DNA binding oligopeptide antibiotic: distamycin A. Exp Cell Res 111: 327–332
Soudek D, Laraya P (1976) C and Q bands in long arm of Y chromosomes: are they identical? Hum Genet 32: 339–341
Zimmer C (1975) Effects of the antibiotic netropsin and distamycin A on the structure and function of nucleic acids. Prog Nucleic Acid Res Mol Biol 15: 285–318
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Lin, M.S., Zhang, A., Wilson, M.G. et al. DA/DAPI-Fluorescent heteromorphism of human Y chromosome. Hum Genet 79, 36–38 (1988). https://doi.org/10.1007/BF00291706
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DOI: https://doi.org/10.1007/BF00291706