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Studies on the influence of liquid holding in Con-A stimulated human peripheral blood lymphocytes on mitosis and X-ray induced chromosome aberrations

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Summary

A method suitable for performance of liquid-holding (LH) experiments with human lymphocytes in vitro is described. The lymphocytes are stimulated with concanavalin A (ConA) and after washing off the ConA with a sugar-containing medium are liquid-held in ConA-free medium for different times, after which they are restimulated with ConA. The BUdR-labeling method was used to determine the proportion of first-, second-, and third-division cells. With this system we were able to show that the frequencies of X-ray-induced chromosomal aberrations are not influenced by liquid holding, supporting the idea that these aberrations are fixed shortly after irradiation by misrepair. This system may prove useful for investigation of specific problems in mutagenicity research, especially those involving mutagens, which induce delayed formation of chromosome aberrations.

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Authors and Affiliations

  1. Genetics Institute, Free University, Arnimallee 5-7, D-1000, Berlin 33

    G. Obe

  2. Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratories, University of Leiden, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands

    A. T. Natarajan & A. den Hertog

  3. Interuniversity Department of Radiation Protection and Radiation Pathology, J. A. Cohen Institute, Rijnsburgerweg, 86, 2333 AD, Leiden, The Netherlands

    G. Obe & A. T. Natarajan

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  1. G. Obe
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  2. A. T. Natarajan
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  3. A. den Hertog
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Obe, G., Natarajan, A.T. & den Hertog, A. Studies on the influence of liquid holding in Con-A stimulated human peripheral blood lymphocytes on mitosis and X-ray induced chromosome aberrations. Hum Genet 54, 385–390 (1980). https://doi.org/10.1007/BF00291586

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  • DOI: https://doi.org/10.1007/BF00291586

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