Summary
The first appearance during mouse development of different allelic products was studied using the supernatant (S-)form of NADP-dependent MDH. Heterozygote offspring were produced by cross-breeding the strains C3H and CBA, and the embryos were taken at various developmental stages. The electrophoretic examination revealed no enzyme activity in unfertilized ova and preimplantation stages. After implantation, an isoenzyme pattern was seen for the first time at day 10 (228 hrs after fertilization), representing the 3 different heteromers of this tetrameric enzyme in binomial distribution. 240 hrs after fertilization, the 5-banded pattern to be expected in the heterozygotes was complete. Measurements of enzyme activity revealed a strong activity increase at about 240 hrs. The results indicate that the S-form of NADP-MDH appears rather late in development and that the two alleles tested at this gene locus commence functioning synchronously.
Zusammenfassung
Das erstmalige Auftreten der Produkte verschiedener Allele eines Genlocus während der Embryogenese wurde am Beispiel der S-NADP-MDH bei der Maus untersucht. Heterozygote Nachkommen der Kreuzung C3HxCBA wurden in verschiedenen Embryonalstadien entnommen und auf Enzymaktivität geprüft. Unberfruchtete Eier sowie präimplantative Stadien zeigen elektrophoretisch keine Aktivität. Im postimplantativen Embryo tritt erstmals am 10. Tag (228 Std nach Befruchtung) ein Elektrophoresemuster auf; die 3 Heteromere des tetrameren Isoenzyms sind in binomialer Verteilung sichtbar. 240 Std nach Befruchtung ist das erwartete 5-Bandenmuster des Heterozygoten komplett. AktivitätsMessungen zeigen einen steilen Anstieg der Enzymaktivität bei 240 Std. Diese Befunde lassen sich dahingehend interpretieren, daß die beiden untersuchten Allele relativ spät in der Embryonalentwicklung synchron ihre Syntheseaktivität aufnehmen.
Similar content being viewed by others
References
Donahue, R. P., Stern, S.: Isocitrate dehydrogenase in mouse embryos: activity and electrophoretic variation. J. Reprod. Fertil. 22, 575–577 (1970).
Gould, E.: Observations on the behaviour of some endogenous enzyme systems in frozenstored fish flesh. In: The Technology of Fish Utilization (Proceedings, F. A. O. Symposium on the significance of fundamental research in utilization of fish, Husum, Germany, 1964), p. 126–128. London: Fishing News (Books) Ltd. 1965.
Henderson, N. S.: Isozymes and genetic control of NADP-malate dehydrogenase in mice. Arch. Biochem. 117, 28–33 (1966).
Hitzeroth, H., Klose, J., Ohno, S., Wolf, U.: Asynchronous activation of parental alleles at the tissue specific gene loci observed on hybrid trout during early development. Biochem. Genet. 1, 287–300 (1968).
Klose, J., Wolf, U.: Transitional hemizygosity of the maternally derived allele at the 6-PGD locus during early development of the Cyprinid fish Rutilus rutilus. Biochem. Genet. 4, 87–92 (1970).
Ohno, S., Stenius, C., Christian, L. C., Harris, C.: Synchronous activation of both parental alleles at the 6-PGD locus of Japanese quail embryos. Biochem. Genet. 2, 197–204 (1968).
Shows, T. B., Chapman, V. M., Ruddle, F. H.: Mitochondrial malate dehydrogenase and malic enzyme: mendelian inherited electrophoretic variants in the mouse. Biochem. Genet. 4, 707–718 (1970).
—, Ruddle, F. H.: Malate dehydrogenase: Evidence for tetrameric structure in Mus musculus. Science 160, 1356 (1968).
Wright, D. A., Moyer, F. H.: Parental influence on lactate dehydrogenase in the early development of hybrid frogs in the genus Rana. J. exp. Zool. 163, 215–230 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Engel, W., Wolf, U. Synchronous activation of the alleles coding for the S-form of the NADP-dependent malate dehydrogenase during mouse embryogenesis. Hum Genet 12, 162–166 (1971). https://doi.org/10.1007/BF00291472
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00291472