Summary
Colchicine-resistant human cells were initially observed in patients exhibiting C-anaphases or tetraploidy in lymphocyte cultures. Cell lines established from these patients displayed cross-resistances to daunomycin, emetine, vinblastine, and vincristine and collateral sensitivity to Xylocaine, showing a pleiotropic phenotype similar to that described in permeability mutants in CHO cells. 3H colchicine uptake and binding assays confirmed a decreased permeability to the drug.
This is a preview of subscription content, log in via an institution to check access.
References
Bech-Hansen NT, Till JE, Ling V (1976) Pleiotropic phenotype of colchicine-resistant CHO cells: cross-resistance and collateral sensitivity. J Cell Physiol 88:23–32
Cabral F, Sobel ME, Gottesman MM (1980) CHO mutants resistant to colchicine, colcemid or griseofulvin have an altered β-tubulin. Cell 20:29–36
Chamla Y, Roumy M, Lassègues M, Battin J (1980) Altered sensitivity to colchicine and PHA in human cultured cells. Hum Genet 53: 249–253
Ling V, Thompson LH (1974) Reduced permeability in CHO cells as a mechanism of resistance to colchicine. J Cell Physiol 83:103–116
Ling V, Baker RM (1978) Dominance of colchicine resistance in hybrid CHO cells. Somat Cell Genet 4: 193–200
Ling V, Aubin JE, Chase A, Sarangi F (1979) Mutants of Chinese Hamster Ovary (CHO) cells with altered colcemid-binding affinity. Cell 18:423–430
Simoni RD, Postma PW (1975) The energetics of bacterial active transport. Ann Rev Biochem 44:523–554
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chamla, Y., Bégueret, J. Colchicine resistance in human cell lines. Pleiotropic phenotype and decreased membrane permeability. Hum Genet 61, 73–75 (1982). https://doi.org/10.1007/BF00291339
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00291339