Summary
Cell hybridization was done between a monolayer of B14-150 Chinese hamster cells and a suspension of either mouse leukemia cells or normal human lymphocytes. Cell contact was obtained by centrifugation of the suspension cells onto the monolayer cells in a culture plate. Cell fusion was done by means of polyethylene glycol (PEG). The optimum time for PEG exposure as well as the yield of hybrid cells differed markedly with the different combinations.
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References
Alexander, P.: Mouse lymphoma cells with different radiosensivities. Nature 192, 572–573 (1961)
Chu, E. H. Y., Brimer, P., Jacobson, K. B., Merriam, E. V.: Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro. Genetics 62, 359–377 (1969)
Davidson, R. L., Gerald, P. S.: Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol. Somat. Cell Genet. 2, 165–176 (1976)
Davidson, R. L., O'Malley, K. A., Wheeler, T. B.: Polyethylene glycol. Induced mammalian cell hybridization. Effect of polyethylene glycol molecular weight and concentration. Somat. Cell Genet. 2, 271–280 (1976)
Littlefield, J. W.: Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145, 709 (1964)
Marshall, C. J.: A method for analysis of chromosomes in hybrid cells employing sequential G-banding and mouse specific C-banding. Exp. Cell Res. 91, 464–469 (1975)
Norwood, T. H., Zeigler, C. J., Martin, G. M.: Dimethyl sulfoxide enhances polyethylene glycol mediated somatic cell fusion. Somat. Cell Genet. 2, 263–270 (1976)
O'Malley, K. A., Davidson, R. L.: A new dimension in suspension fusion techniques with polyethylene glycol. Somat. Cell Genet. 3, 441–448 (1977)
Pontecorvo, G.: Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment. Somat. Cell Genet. 1, 397–400 (1975)
Pontecorvo, G., Riddle, P. N., Hales, A.: Time and mode of fusion of human fibroblasts treated with polyethylene glycol (PEG). Nature 265, 257–258 (1977)
Russel, W. C., Newman, C., Williamson, D. H.: A simple cytochemical technique for demonstration of DNA in cells infected with myoplasmas and viruses. Nature 253, 461–462 (1975)
Sumner, A. T., Evans, H. J., Buckland, R. A.: New technique for distinguishing between human chromosomes. Nature [New Biol.] 232, 31–32 (1971)
Thorsby, E., Bratlie, A.: A rapid method for preparation of pure lymphocyte suspensions. In: Histocompatibility testing 1970, pp. 655–656. Copenhagen: Munksgaard 1970
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Anders, G.J.P.A., Wierda, J., Nienhaus, A.J. et al. Time and cell systems as variables in fusion experiments with polyethylene glycol. Hum Genet 42, 319–322 (1978). https://doi.org/10.1007/BF00291313
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DOI: https://doi.org/10.1007/BF00291313