Archives of Microbiology

, Volume 155, Issue 1, pp 52–55 | Cite as

Enzymatic evidence for involvement of the methylmalonyl-CoA pathway in propionate oxidation by Syntrophobacter wolinii

  • Frans P. Houwen
  • Jeannette Plokker
  • Alfons J. M. Stams
  • Alexander J. B. Zehnder
Original Papers


Enzyme measurements were carried out with crude cell-free extracts of the propionate oxidizing coculture of Syntrophobacter wolinii and Desulfovibrio G11. Using cell-free extracts of a pure culture of Desulfovibrio G11 as a blank, most of the enzymes involved in the methylmalonyl-CoA pathway for propionate oxidation, including a propionyl-CoA: oxaloacetate transcarboxylase, were demonstrated in S. wolinii.

Key words

Syntrophobacter wolinii Propionate oxidation Syntrophic coculture Propionyl-CoA: oxaloacetate transcarboxylase Methylmalonyl-CoA pathway Desulfovibrio 


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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Frans P. Houwen
    • 1
  • Jeannette Plokker
    • 1
  • Alfons J. M. Stams
    • 1
  • Alexander J. B. Zehnder
    • 1
  1. 1.Department of MicrobiologyAgricultural University of WageningenWageningenThe Netherlands

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