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Archives of Microbiology

, Volume 155, Issue 1, pp 46–51 | Cite as

Propionate acts as carboxylic group acceptor in aspartate fermentation by Propionibacterium freudenreichii

  • Bettina Rosner
  • Bernhard Schink
Original Papers

Abstract

Cells of Propionibacterium freudenreichii ssp. shermanii and ssp. freudenreichii did not show significant growth or product formation in a mineral medium with 10 mM aspartate or 10 mM fumarate, vitamins, and a small amount (0.05% w/v) of yeast extract. In the presence of added propionate, growth with aspartate or fumarate was possible, and depended strictly on the amount of propionate provided, according to the equation: 3 aspartate + propionate → 3 succinate + acetate + CO2+3 NH3. Cocultures of P. freudenreichii with the succinate-decarboxylating strain Ft2 converted 3 aspartate stoichiometrically to acetate and 2 propionate. High activity of methylmalonyl-CoA: pyruvate transcarboxylase, and lack of methylmalonyl-CoA decarboxylase and oxaloacetate decarboxylase activity in cell-free extracts of aspartate-grown cells indicated that failure to use aspartate as sole substrate was due to the inability of these strains to catalyze a net decarboxylation of C4-dicarboxylic acids.

Key words

Aspartate fermentation Transcarboxylase Decarboxylases Anaerobic cocultures Propionate fermentation Succinate fermentation Propionibacterium freudenreichii 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Bettina Rosner
    • 1
  • Bernhard Schink
    • 1
  1. 1.Lehrstuhl Mikrobiologie I der Eberhard-Karls-UniversitätTübingenFederal Republic of Germany

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