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Archives of Microbiology

, Volume 155, Issue 1, pp 18–21 | Cite as

Several new substrates for Desulfovibrio vulgaris strain Marburg and a spontaneous mutant from it

  • Kazuhiro Tanaka
Original Papers

Abstract

The ability of Desulfovibrio vulgaris strain Marburg (DSM 2119) to oxidize alcohols was surveyed in the presence and absence of hydrogen-scavenging anaerobes, Acetobacterium woodii and Methanospirillum hungatei. In the presence of sulfate, D. vulgaris grew not only on ethanol, 1-propanol, and 1-butanol, but also on isobutanol, 1-pentanol, ethyleneglycol, and 1,3-propanediol. Metabolism of these alcohols was simple oxidation to the corresponding acids, except with the last two substrates: ethyleneglycol was oxidized to glycolate plus acetate, 1,3-propanediol to 3-hydroxypropionate plus acetate. Experimental evidence was obtained, suggesting that 2-methoxyethanol was not utilized by all the cells of strain marburg, but by a spontaneous mutant. 2-Methoxyethanol was oxidized to methoxyacetate by the mutant. Co-culture of strain Marburg plus A. woodii grew on ethanol, 1-propanol, 1-butanol, and 1,3-propanediol in the absence of sulfate. Co-culture of strain Marburg plus M. hungatei grew on ethanol, 1-propanol, and 1-butanol, but not on ethyleneglycol and 1,3-propanediol, Co-culture of the mutant plus A. woodii or M. hungatei did not grow on 2-methoxyethanol.

Key words

Desulfovibrio vulgaris Acetobacterium woodii Methanospirillum hungatei Oxidation Alcohol 2-Methoxyethanol Spontaneous mutant Anaerobic Syntrophic Interspecies hydrogen transfer 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Kazuhiro Tanaka
    • 1
  1. 1.Fermentation Research InstituteTsukubaJapan

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