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Archives of Microbiology

, Volume 155, Issue 1, pp 13–17 | Cite as

ε-caprolactam, a new powerful inducer for the formation of Rhodococcus rhodochrous J1 nitrilase

  • Toru Nagasawa
  • Tetsuji Nakamura
  • Hideaki Yamada
Original Papers

Abstract

We found that ε-caprolactam is a new powerful inducer for the formation of Rhodococcus rhodochrous J1 nitrilase. When Rhodococcus rhodochrous J1 cells were cultivated at 28°C for 120 h in a nutrient medium supplemented with 0.5% (w/v) ε-caprolactam, an enormous amount of nitrilase was formed in the cells which corresponded to approximately 30% of all soluble protein. The level of ε-caprolactam in the culture broth barely decreased in the course of cultivation. γ-Butyrolactam and δ-valerolactam also caused effective induction. The induction of nitrilase formation by ε-caprolactam was also observed in some other Rhodococcus strains.

Key words

Nitrilase ε-Caprolactam Rhodococcus rhodochrous J1 Induction 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Toru Nagasawa
    • 1
  • Tetsuji Nakamura
    • 2
  • Hideaki Yamada
    • 1
  1. 1.Department of Agricultural ChemistryKyoto UniversitySakyo-ku, KyotoJapan
  2. 2.Nitto Chemical IndustryTsurumi-ku, YokohamaJapan

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