Summary
Chromosome arrangement on the metaphase plate was studied in 168 colchicine and hypotonic treated human cells where each chromosome was identified by quinacrine fluorescent staining. Large chromosomes appeared to lie at random within the cell with respect to the center of the metaphase plate, while smaller chromosomes tended to be centrally located. Angle subtended at the center of a cell was used as a measure of interchromosomal distance which was independent of distance from the center. No tendency for non-random association of homologous or non-homologous chromosomes could be demonstrated except for the well-known association of acrocentric chromosomes.
Zusammenfassung
Die Anordnung der Chromosomen auf der Metaphaseplatte von 168 mit Colchicin und hypotonischem Schock behandelten menschlichen Zellen wurde nach Identifikation der Chromosomen durch Quinancrin-Fluorescenzfärbung untersucht. Große Chromosomen waren in bezug auf die Mitte der Metaphase zufällig verteilt, während kleinere Chromosomen eine Tendenz zu mehr zentraler Lagerung zeigten. Die Distanz zwischen den Chromosomen war unabhängig von dem Abstand zum Zentrum. Es fand sich keine Tendenz für nichtzufällige Assoziationen homologer oder nichthomologer Chromosomen mit Ausnahme der bereits gut bekannten Assoziation akrozentrischer Chromosomen.
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Warburton, D., Naylor, A.F. & Warburton, F.E. Spatial relations of human chromosomes identified by quinacrine fluorescence at metaphase. Hum Genet 18, 297–306 (1973). https://doi.org/10.1007/BF00291126
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DOI: https://doi.org/10.1007/BF00291126